DNA ladders, loading buffers and gel stains
SafeGel green stain
10,000x fluorescent nucleic acid stain, excitable with blue light and UV
- Cat. No.
- M3191
- Formats
- 4 formats
- Manufacturer
A nucleic acid stain supplied as a 10,000x concentrate in water, for visualising double- and single-stranded DNA and RNA in agarose and polyacrylamide gels as a safer alternative to ethidium bromide. It is excited by blue-green light (480-530 nm) but also works on standard UV transilluminators, and it is more sensitive than ethidium bromide with no destaining needed. Safety testing found it non-toxic and non-mutagenic at working concentrations, and it can go into normal waste in line with local rules.
Key features
Safer than ethidium bromide
Non-toxic, non-mutagenic and non-hazardous at the recommended working concentrations.
Blue light or UV
Optimal excitation at 480-530 nm, also usable on UV transilluminators (254-366 nm).
High sensitivity
More sensitive than ethidium bromide without destaining; about twice as sensitive for dsDNA as for ssDNA and RNA.
Stable at room temperature
Stored at +15/+30 °C protected from light and shipped without cooling.
Applications
- Staining DNA and RNA in agarose gels
- Post-staining of polyacrylamide gels
- Formaldehyde RNA gels (pre-staining)
- DGGE, EMSA and PFGE gels (post-staining)
- Checking transfers in blotting
- DNA recovery for digestion, cloning, ligation and sequencing
Technical details
| Concentration | 10,000x in water |
|---|---|
| Excitation | Blue-green light 480-530 nm; also UV 254-366 nm |
| Nucleic acids detected | dsDNA, ssDNA, RNA |
| Methods | In-well staining (recommended, 20x solution with the loading buffer), pre-staining or post-staining |
| Usage notes | Requires a separate loading buffer; SDS in the loading buffer can cause smeared bands |
| Compatibility | Restriction digestion, Southern blotting, cloning, ligation, sequencing |
| Storage | +15/+30 °C protected from light; if it precipitates, warm to 45-50 °C for 2 minutes and shake |
| Shipping | Ambient temperature |
| Stability | Stable long term at room temperature |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.