DNA ladders, loading buffers and gel stains
SafeGel red stain
10,000x fluorescent nucleic acid stain, imaged with the same filters as ethidium bromide
- Cat. No.
- M3193
- Formats
- 4 formats
- Manufacturer
A nucleic acid stain supplied as a 10,000x concentrate in water that detects double- and single-stranded DNA and RNA in agarose and polyacrylamide gels. Its spectrum is virtually the same as ethidium bromide, so the same UV transilluminators, filters and camera settings apply, with higher sensitivity and no destaining. Ames tests by a third-party laboratory found it non-mutagenic at staining concentrations, and it does not penetrate latex gloves or cell membranes.
Key features
No change to the imaging set-up
Spectrum matches ethidium bromide: same filter, same settings.
Favourable safety profile
Non-mutagenic in the Ames test, does not penetrate cell membranes and can go into normal waste in line with local rules.
High sensitivity
More sensitive than ethidium bromide, especially on low molecular weight fragments, with no destaining.
Flexible protocols
In-well staining, precast gels or post-staining; the post-staining solution can be reused 2-3 times.
Applications
- Staining DNA and RNA in agarose gels
- Post-staining of polyacrylamide gels
- Formaldehyde RNA gels
- DGGE, EMSA and PFGE gels
- Southern and Northern blotting
- DNA recovery for digestion, cloning, ligation, sequencing and PCR
Technical details
| Concentration | 10,000x in water |
|---|---|
| Excitation | Standard UV transilluminator (302 or 312 nm); ethidium bromide filter |
| Nucleic acids detected | dsDNA (about 2 times more sensitive), ssDNA, RNA |
| Precast gels | 1:10,000 dilution (5 µL in 50 mL of gel at 50-55 °C); not suitable for polyacrylamide gels |
| Post-staining | 1x or 3x solution (1:3,300-1:10,000 dilution), optional 0.1 M NaCl, about 30 minutes (30-60 minutes for polyacrylamide) |
| In-well staining | 20x solution with the loading buffer, 1-2 µL per 4 µL of sample |
| Safety | Non-mutagenic in the Ames test at staining concentrations |
| Storage | Room temperature in the dark or +2/+8 °C; if it precipitates, warm to 45-50 °C for 2 minutes and vortex |
| Shipping | Ambient temperature |
| Stability | 1x or 3x working solutions stable for at least one year at room temperature in the dark |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.