Dehydrated culture media
Standard Nutrient Agar I
For the cultivation and enumeration of fastidious bacteria.
- Cat. No.
- LC-1177
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Standard Nutrient Agar I is a medium suitable for the cultivation and enumeration of fastidious bacteria. The addition of blood, ascites fluid or serum makes it also suitable to cultivate streptococci, pneumococci and other microorganisms. It is employed for the enumeration and isolation of bacteria, and also as a high-grade base for preparing special culture media.
Key features
Applications
Non-selective enumeration of fastidious microorganisms.
Industry
General cultivation.
Preparation
37 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
The peptones present in the formula provide nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.5 ± 0.2 |
| Amount per litre | 37 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 12 |
| Dextrose | 1 |
| Peptone | 15 |
| Sodium chloride | 6 |
| Yeast extract | 3 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 37 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Incubate the Petri dishes aerobically at 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Shigella flexneriATCC 12022 | Good growth |
| Listeria monocytogenes 4bATCC 13932 | Good growth |
| Streptococcus pyogenesATCC 19615 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
Bibliography
- American Public Health Association 1917. Standard methods of water analysis, 3rd Ed. American Public Health Association, Washington D.C.
- American Public Health Association 1923. Standard methods of water analysis, 5th Ed. American Public Health Association, Washington D.C.
- Eaton, A.D., L. S. Clesceri, and A. E. Greenberg (Ed). 1995. Standard methods for the examination of water and wastewater, 19th ed. American Public Health Association, D.C.
- Vanderzant C., and D. F. Splittstoesser (Ed). 1992. Compendium of methods for the microbiological examination of foods, 3rd ed. American Public Health Association, D.C.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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