Dehydrated culture media
Standard Count Agar
For total microbial plate count in milk, milk products, water and waste water.
- Cat. No.
- LC-1178
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Standard Count Agar is recommended to enumerate the aerobic bacteria in milk, dairy products, drinking water and wastewater. It is especially suitable to prepare special culture media that need to be rich in nutrients.
Key features
Applications
Selective enumeration of mesophilic aerobes.
Industry
Dairy products, water.
Preparation
25 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Casein and meat peptone provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.2 ± 0.2 |
| Amount per litre | 25 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 12 |
| Casein peptone | 5 |
| Meat peptone | 3 |
| Sodium chloride | 5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 25 grams of the medium in 1 liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes. Cool to 50 °C, mix well and dispense into plates.
Instructions for use
- Inoculate the plates by the surface plating or pour plate techniques.
- Incubate at a temperature of 35 ± 2 °C for 18-24 hours.
- Count the developed colonies.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Bacillus cereusATCC 11778 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
| Pseudomonas aeruginosaATCC 27853 | Good growth |
Bibliography
- Downes F. P. and Ito K., (Eds.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th Ed., APHA, Washington, D.C.
- Standard Methods for the Examination of Dairy Products, 13th Ed. APHA, 1972. American Public Health Association.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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