Dehydrated culture media
TAT Broth Base (CPLP)
For proliferation and regeneration of microorganisms from a variety of samples, e.g. from cosmetics, and samples that contain antimicrobial reagents.
- Cat. No.
- LC-1397
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
TAT Broth Base (CPLP) is used as a diluent of pharmaceutical, cosmetic and other raw material samples.
Key features
Applications
Enrichment (general use).
Industry
Pharmaceutical/veterinary.
Preparation
25 g in 960 mL of distilled water; boil for one minute, add 40 mL of polysorbate, autoclave at 121 °C for 15 minutes.
Principles and uses
Casein pancreatic digest provides nitrogen, vitamins, minerals and amino acids essential for growth. The high concentration of casein peptone allows the development of spores of all microorganisms, including the injured ones. Soy lecithin and polysorbate neutralize preservatives included in cosmetic and pharmaceutical products, allowing microorganism growth.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Beige, slightly opalescent |
| Final pH (25 °C) | 7.1 ± 0.2 |
| Amount | 25 g in 960 mL |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Pancreatic digest of casein | 20 |
| Soy lecithin | 5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 25 grams in 960 mL of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Add 40 mL of polysorbate. Distribute into appropriate containers and autoclave at 121 °C for 15 minutes.
Instructions for use
Inoculate and incubate at 35 ± 2 °C for 24 hours; negative tubes must be kept for 7 days. Tubes or bottles exhibiting growth should be subcultured for identification.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 24 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Moderate growth |
| Clostridium perfringensATCC 13124 | Good growth |
| Streptococcus pyogenesATCC 19615 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
| Pseudomonas aeruginosaATCC 27853 | Good growth |
Bibliography
- FDA Bacteriological Analytical Manual (BAM) 1995. Microbiological Methods for cosmetics.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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