Dehydrated culture media
Acetamide Agar
For the differentiation of non-fermentative Gram-negative bacteria, in particular Pseudomonas aeruginosa.
- Cat. No.
- LC-1391
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Acetamide Agar is used to determine the ability of non-fermenting Gram-negative bacteria to deaminate the acetamide. The deamination of the acetamide produces ammonia which increases the pH of the medium. The resulting alkalinization is shown by a color change of the phenol red from yellow-orange to purple-red.
Key features
Applications
Differentiation of non-fermentative Gram-negative bacteria and Pseudomonas aeruginosa.
Industry
Cosmetics, water.
Preparation
24.7 g in one liter of distilled water; boil for one minute, dispense into tubes and sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Pseudomonas aeruginosa is an opportunistic pathogen for humans, capable of growing in water with a low concentration of nutrients. This is why natural mineral water and spring water are Pseudomonas aeruginosa-free at the time of their commercialization. This microorganism can also be found in swimming pool water.
Acetamide deamination is accomplished by Pseudomonas aeruginosa, Pseudomonas acidovorans, Group III (Achromobacter xylosoxidans), and Alcaligenes odorans.
Acetamide is a carbon source. Dextrose is a fermentable carbohydrate providing carbon and energy, the potassium salts have a high buffering capacity. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Phenol red is a pH indicator and bacteriological agar is the solidifying agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Pink-orange |
| Color of the prepared medium | Yellow-orange |
| Final pH (25 °C) | 6.3 ± 0.2 |
| Amount per litre | 24.7 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Acetamide | 3 |
| Bacteriological agar | 15 |
| Dextrose | 0.2 |
| Phenol red | 0.03 |
| Potassium dihydrogen phosphate | 1 |
| Sodium chloride | 5 |
| Yeast extract | 0.5 |
Preparation and use
Preparation
Suspend 24.7 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into tubes and sterilize in autoclave at 121 °C for 15 minutes. Allow to cool in a slanted position in order to obtain butts of 1.5-2.0 cm depth.
Instructions for use
- Inoculate and incubate at a temperature of 35 ± 2 °C for 24-48 hours.
- A positive reaction turns the medium an intense purple-red.
- P. aeruginosa is confirmed by positive asparagine and acetamide tests.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 24-48 h.
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Escherichia coliATCC 25922 | Good growth | No color change of the medium to purple-red |
| Pseudomonas aeruginosaATCC 27853 | Good growth | Color change of the medium to purple-red |
| Proteus mirabilisATCC 29906 | Good growth | No color change of the medium to purple-red |
| Pseudomonas aeruginosaATCC 9027 | Good growth | Color change of the medium to purple-red |
Bibliography
- Gilardi. 1974. Antonie van Leeuwenhoek. J. Microbiol. Serol. 39:229.
- Buhlmann, Vischer and Bruhin. 1961. J. Bacteriol. 82:787.
- Clesceri, Greenberg and Eaton (ed.) 1998. Standard methods for the examination of water and wastewater, 20th ed. American Public Health Association, Washington, D.C.
- Murray, Baron, Pfaller, Tenover and Yolken (ed.). 1999. Manual of clinical microbiology, 7th ed. American Society for Microbiology, Washington, D.C.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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