Dehydrated culture media
Vegetable Peptone Broth (TSB Vegetable)
Universal culture medium without inhibitors and broad-spectrum indicators.
- Cat. No.
- LC-1380
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Vegetable Peptone Broth (TSB Vegetable) is a non-animal Trypticasein Soy Broth medium very rich in nutrients for general use in microbiological laboratories.
Key features
Applications
Diluent and enrichment (general use).
Industry
Cosmetics, food, general cultivation, pharmaceutical/veterinary.
Preparation
30 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
It supports the abundant growth of fastidious organisms such as pneumococci, streptococci, neisseriae, etc. The medium is used frequently in many procedures of microbiology. It is used for the isolation and sensitivity testing of all types of pathogens, and for the production of antigens for agglutination and serological tests. The peptone used in this broth is from non-animal origin and conforms to the requirements of the pharmaceutical industry and biotechnology.
Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate is a buffering agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 30 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 2.5 |
| Dipotassium phosphate | 2.5 |
| Sodium chloride | 5 |
| Vegetable peptone | 20 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 30 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Take the inoculum with a sterile loop.
- Submerge the loop into the medium and shake gently.
- Incubate at 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Aspergillus brasiliensisATCC 16404 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
| Streptococcus pneumoniaeATCC 6305 | Good growth |
| Staphylococcus aureusATCC 6538 | Good growth |
| Bacillus subtilisATCC 6633 | Good growth |
Bibliography
- Deutsches Arzneibuch, 10. Auflage; European Pharmacopeia II, United States Pharmacopeia XXIII.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
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