Dehydrated culture media
Tryptose Phosphate Broth II
For the cultivation of fastidious microorganisms.
- Cat. No.
- LC-1294
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Standards
- BAM
Tryptose Phosphate Broth II is recommended for the cultivation of fastidious and pathogenic microorganisms as well as antibiotic resistance and sensitivity tests by the serial tube dilution technique. If desired, the addition of 0.1 to 0.2% agar to the broth enhances the growing of anaerobic fastidious microorganisms by delaying the dispersion and diffusion of CO₂. The low concentration of agar provides ideal condition for the aerobic growing in the upper part of the tubes and anaerobic and micro-aerobic conditions growing in the lower part of the tube.
Key features
Applications
Antibiotic assay and selective enrichment of fastidious microorganisms.
Industry
General cultivation.
Standards
BAM.
Preparation
29.5 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. The buffering capacity is provided by the disodium phosphate.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Clear beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 29.5 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 2 |
| Disodium phosphate | 2.5 |
| Sodium chloride | 5 |
| Tryptose | 20 |
Preparation and use
Preparation
Suspend 29.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution and dispense into the tubes. Sterilize in autoclave at 121 °C for 15 minutes. While preparing this medium it is important to avoid overheating and to distribute it into tubes before sterilization.
Instructions for use
Inoculate and incubate at a temperature of 37 ± 2 °C for 24-38 hours.
Quality control
Microbiological test
Incubation conditions: 37 ± 2 °C / 24-38 h.
| Microorganism | Specification |
|---|---|
| Staphylococcus epidermidisATCC 12228 | Good growth |
| Neisseria meningitidisATCC 13090 | Good growth |
| Streptococcus pyogenesATCC 19615 | Good growth |
| Streptococcus pneumoniaeATCC 6305 | Good growth |
Bibliography
- Gray, M.L., Stafseth, H.J. and Thorp, F. Jr.: The use of potassium tellurite, sodium azide and acetic acid in a selective medium for the isolation of Listeria monocytogenes. J. Bact., 59: 443-444 (1950).
- Waisbren, B.A., M.S. Carr and J. Dunnett, 195. The tube dilution method of determining bacterial sensitivity to antibiotics. Am. J. Clin. Pathol. 21:884.
- Ginsberg, Gold, and Jordan, 1955. Proc. Soc. Exp. Biol. Med. 89.66.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.