Dehydrated culture media
Standard Nutrient Broth I
For the enrichment and cultivation of fastidious bacteria.
- Cat. No.
- LC-1286
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Standard Nutrient Broth I is a medium suitable for the cultivation of fastidious bacteria. The addition of blood, ascitic fluid or serum makes it also suitable to cultivate streptococci, pneumococci and other microorganisms. It is used for the enumeration, isolation and enrichment of bacteria, and also as a high grade base for the preparation of special culture media.
Key features
Applications
Enrichment of fastidious microorganisms.
Industry
General cultivation.
Preparation
25 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Peptone present in the formula provides nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the fermentable carbohydrate providing carbon and energy. Yeast extract is a source of vitamins, particularly of the B-group. Sodium chloride supplies essential electrolytes for transport and osmotic balance.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 7.5 ± 0.2 |
| Amount per litre | 25 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 1 |
| Peptone | 15 |
| Sodium chloride | 6 |
| Yeast extract | 3 |
Preparation and use
Preparation
Suspend 25 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Inoculate the tubes of Standard Nutrient Broth I with the sample.
- Incubate at a temperature of 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Shigella flexneriATCC 12022 | Good growth |
| Listeria monocytogenes 4bATCC 13932 | Good growth |
| Streptococcus pyogenesATCC 19615 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
| Streptococcus pneumoniaeATCC 6305 | Good growth |
Bibliography
- Eaton, A.D., L.S. Clesceri, and A.E. Greenberg (Ed). 1995. Standard methods for the examination of water and wastewater, 19th ed. American Public Health Association, D.C.
- Vanderzant C., and D.F. Splittstoesser (Ed). 1992. Compendium of methods for the microbiological examination of foods, 3rd ed. American Public Health Association, D.C.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.