Dehydrated culture media
Sabouraud Dextrose Broth + Chloramphenicol
For the selective cultivation and isolation of yeasts and molds.
- Cat. No.
- LC-1284
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Sabouraud Chloramphenicol Dextrose Broth is a selective medium that can be used for the cultivation of yeasts, molds and aciduric microorganisms. It is used for determining the microbial and fungal content of cosmetics and for the mycological evaluation of food.
Key features
Applications
Selective enrichment and selective isolation of pathogenic fungi and yeasts.
Industry
Cosmetics, food.
Preparation
30.5 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Dextrose is the fermentable carbohydrate providing carbon and energy. Peptone mixture provides nitrogen, vitamins, minerals and amino acids essential for growth. The dextrose concentration and acidic pH make this medium selective for fungi.
This medium is a modification of the Dextrose Broth described by Sabouraud (LC-1205), with the addition of chloramphenicol, which inhibits the great majority of bacterial contaminants.
Chloramphenicol is an antibiotic which aids in isolating pathogenic fungi from heavily contaminated material, as it inhibits most contaminating bacteria. It is a recommended antibiotic for this type of media due to its heat stability and wide bacterial spectrum.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 5.6 ± 0.2 |
| Amount per litre | 30.5 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 20 |
| Chloramphenicol | 0.5 |
| Peptone mixture | 10 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 30.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes. DO NOT OVERHEAT.
Instructions for use
Inoculate and incubate at a temperature of 30 °C for 3-7 days.
Quality control
Microbiological test
Incubation conditions: 30 °C / 3-7 days.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 2091 | Good growth |
| Escherichia coliATCC 25922 | Total inhibition |
| Staphylococcus aureusATCC 25923 | Total inhibition |
| Candida tropicalisATCC 750 | Good growth |
Bibliography
- Sabouraud R. 1892. Ann. Dermatol. Syphilol. 3:1061.
- Jarett, L., and A.C. Sonnenwirth (ed) 1980. Gradwohl's clinical laboratory methods and diagnosis, 8th ed. CV Mosby.
- Curry, A. S., J. G. Graf, and G. N. McEwen, Jr. (ed) 1993. CTFA Microbiology Guidelines. The Cosmetic, Toiletry, and Fragrance Association, Washington, D.C.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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