TSA + Penase Triple Wrapped. Irradiated

Description

This widely used culture medium contains soya and casein peptones in proven proportions to support the growth of most microorganisms, including some very fastidious ones. It has been formulated according to the harmonized method of pharmacopoeias and ISO standards and is regularly used in routine diagnostic work for its reliability in the morphological aspects and reproducibility of the results. Penicillinase ensures the inactivation of penicillins or cephalosporins that may be present in the air or surfaces to be sampled, allowing the growth of organisms sensitive to these antibiotics.

Attention: Petri plates are used for monitoring the microbiological contamination of surface and air inside cleanrooms, isolators, RABS, food industries and hospitals. The double/triple irradiated wrapping ensures that the package itself doesn't contaminate the environment as the first wrapper is removed just before entering the clean area.

Technique

In the microbiological control of cleaning and disinfection of smooth surfaces in the "clean zones", the contact plates are used as a plug or copy-pad which acts simultaneously as a sampler and culture medium to be incubated, without other intermediate operations. However, if the surfaces to be sampled are rough, it is preferable to delimit a surface (e.g. 5 x 5 cm) and thoroughly rub it with a moist, sterile swab that will be used immediately to inoculate the surface of the plate.

At the time of using the plates, the outer shell is removed, the plate is opened and the surface of the culture medium is inoculated with the swab. The plate is covered to avoid contamination and labeled appropriately with the data (place, date and time) of the sample. The inoculated plates are incubated at 30-35 °C for 24-72 h (bacteria) and 3-5 days for fungi (yeasts and molds). Examined daily.

Attention: Petri plates are used for monitoring the microbiological contamination of surface and air inside cleanrooms, isolators, RABS, food industries and hospitals. The double/triple irradiated wrapping ensures that the package itself doesn't contaminate the environment as the first wrapper is removed just before entering the clean area.

Wrapping resistant to hydrogen peroxide vapor penetration.

Penicillinase to inactivate 10,000,000 IU PenG/L/min.

Physical/chemical control

Microbiological control

Control post addition of penicillin, according to harmonized pharmacopoeial monographs and test methods.

Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.

Aerobiosis. Incubation at 30-35 °C. Read after 18-24 h to 72 h for bacteria and 3-5 days for fungi.

Sterility control

Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.

Check at 7 days after incubation in same conditions.

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• COLIPA (1997) Guidelines on Microbial Quality Management (MQM). Brussels.
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• ISO 11731 Standard (2017) Water Quality - Enumeration of Legionella.
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• ISO 18415 Standard (2017) Cosmetics - Microbiology - Detection of specified and non-specified microorganisms.
• ISO 21149 Standard (2017) Cosmetics - Microbiology - Enumeration and detection of aerobic mesophilic bacteria.
• ISO 21150 Standard (2015) Cosmetics - Microbiology - Detection of Escherichia coli.
• ISO 22717 Standard (2015) Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa.
• ISO 22718 Standard (2015) Cosmetics - Microbiology - Detection of Staphylococcus aureus.
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