Ready-to-use media
Urea Indol Broth ISO
Liquid medium for determination of urease and indole test.
- Cat. No.
- LC-4212
- Category
- Ready-to-use media
- Format
- 20 tubes
- ISO 17025 quality control
- Yes
- Storage
- 8 to 14 °C
- Minimum guaranteed shelf life
- 4 months
Ready-to-use media in plates, contact plates, tubes, flasks and bags.
Key features
Applications
Confirmation of enterobacteria.
Industry
Food.
Presentation
20 tubes, 16 x 113 mm, with 2 ± 0.1 mL.
Packaging
16 x 113 mm glass tubes, ink labelled, metal non-injectable cap. 20 tubes per box.
Description and technique
Inoculate with a swab or a loop with the isolated colony, using one tube per isolate.
Incubate 21 ± 3 h at 37 ± 1 °C before reading.
Urease-positive microorganisms turn the medium from clear yellowish to clear pink due to the pH indicator phenol red change, because of the production of alkaline metabolites.
The addition of Kovac's Reagent to show indole production due to tryptophan hydrolysis must be done only after having read the urease reaction. Formation of a red ring indicates indole presence.
- E. coli ATCC 25922: urease (-), indole (+).
- P. mirabilis ATCC 14253: urease + (6 hours), indole (-).
- P. hauseri ATCC 13315: urease (+), indole (+).
Presentation
| Presentation | 20 tubes, 16 x 113 mm |
|---|---|
| Filling | 2 ± 0.1 mL |
| Packaging | 16 x 113 mm glass tubes, ink labelled, metal non-injectable cap. 20 tubes per box. |
Composition
| Ingredient | g/L |
|---|---|
| Peptone | 1.000 |
| D(+) Glucose | 1.000 |
| Sodium chloride | 5.000 |
| Potassium dihydrogen phosphate | 0.800 |
| Dipotassium hydrogen phosphate | 1.200 |
| Phenol red | 0.012 |
| Tryptophan | 1.000 |
| Urea | 20.000 |
Quality control
Physical/chemical control
| Color | Yellow-orange |
|---|---|
| pH | 6.8 ± 0.2 at 25 °C |
Microbiological control
Impregnate the swab with a recently isolated pure culture.
Inoculate with loop/swab with pure isolates to get a thick suspension.
Aerobiosis. Incubation at 37 ± 1 °C, reading after 24 ± 3 h.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 25922, WDCM 00013 | Good - negative urease - positive indole |
| Proteus hauseriATCC 13315 | Good - positive urease - positive indole |
| Proteus mirabilisATCC 43071 | Good - positive urease - negative indole |
| Salmonella typhimuriumATCC 14028, WDCM 00031 | Good - negative urease - negative indole |
| Enterobacter aerogenesATCC 13048, WDCM 00175 | Good - negative urease - negative indole |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. & L.C. PARK (1993) Handbook of Microbiological Media. CRC Press Inc. London.
- DOWNES, F.P. & K. ITO (2001) Compendium of Methods for the Microbiological Examination of Foods. 4th ed. APHA. Washington.
- COWAN & STEEL'S (1993) Manual for identification of medical bacteria. 3rd. Edition edited and revised by G.I. Barrow and R.K.A. Feltham. Cambridge University Press, Cambridge USA.
- FDA (Food and Drug Administration) (1998) Bacteriological Analytical Manual. 8th ed. Rev. A. AOAC International. Gaithersburg. MD. USA.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- IN BALOWS, A., et al. (1991) Chapter 122. Manual of Clinical Microbiology. 6th Edition. American Society for Microbiology, Washington, D.C.
- PASCUAL ANDERSON, Mª R. (1992) Microbiología Alimentaria. Díaz de Santos. S.A. Madrid.
- RUSTIGIAN, R. & C.A. STUART (1941) Decomposition of urea by Proteus. Proc. Soc. Exp. Biol. Med. 47:108.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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