PCR accessories
10X PCR Buffer S, incomplete (without MgCl2)
KCl-based 10x PCR buffer without MgCl2, for high-specificity routine PCR
- Cat. No.
- M3453
- Category
- PCR accessories
- Formats
- 1 format
- Manufacturer
A 10x reaction buffer based on Tris-HCl and KCl with Triton X-100, free of MgCl2 and ammonium sulphate, to be completed with the magnesium level that best suits the reaction. It is the standard buffer for the Taq DNA polymerase of the same range and works with all polymerases in the range. It increases PCR specificity, giving sharper bands and fewer side products.
Key features
Magnesium of your choice
No MgCl2: titrate between 0 and 3.0 mM final for best results.
High specificity
Sharper bands and fewer side products.
Nuclease-free
DNases, RNases and proteases not detectable.
Long shelf life
Stable for more than 24 months at -20 °C.
Applications
- High-specificity routine PCR
- Optimising the magnesium concentration
- PCR, qPCR, RT-PCR and RT-qPCR
Technical details
| Concentration | 10x |
|---|---|
| Composition (10x) | 100 mM Tris-HCl, 500 mM KCl, 1% Triton X-100; no MgCl2 and no (NH4)2SO4 |
| Recommended MgCl2 | Titrate between 0 and 3.0 mM final |
| Compatibility | Taq DNA polymerase and other polymerases of the range |
| Contaminants | DNases, RNases and proteases not detectable |
| Storage | -20 °C; vortex before use |
| Shipping | Ambient temperature |
| Stability | More than 24 months at -20 °C |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.