Dehydrated culture media
Agar Base Aeromonas (RYAN)
For the selective isolation of Aeromonas hydrophila from clinical and environmental samples when ampicillin is used as a selective supplement.
- Cat. No.
- LC-1370
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Aeromonas Agar Base (RYAN) is based on the formulation of Ryan. It is a modification of the XLD Medium that has been designed to improve the count and isolation of Aeromonas in clinical and environmental samples. The medium is better than other media in the detection of Aeromonas in water, bottled water and food (meat, fish, etc.).
Supplement to be added: Ampicillin Supplement (Aeromonas) LC-6052
Key features
Applications
Selective isolation of Aeromonas.
Industry
Water.
Preparation
28.1 g in 500 mL of distilled water; boil for one minute, avoid overheating, do not autoclave.
Principles and uses
Proteose peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. L-lysine and L-arginine provide nitrogen, sulfur and trace elements. Yeast extract is a source of vitamins, particularly the B-group. Inositol, lactose, sorbitol and xylose are the carbohydrate substrates. Sodium thiosulfate provides sulphur, and ferric ammonium citrate is the indicator for H₂S production. H₂S positive colonies have a black center. The mixed indicators bromothymol blue and thymol blue change their color to yellow when acid is formed. Sodium chloride supplies essential electrolytes for transport and osmotic balance and bile salts are inhibitors of Gram-positive organisms.
Aeromonas are aquatic organisms, fresh water bacteria that were identified during the last century as responsible for infectious processes in aquatic animals: amphibians, reptiles, fish, snails and others. Aeromonas also cause intestinal tract diseases. Infections are contracted frequently due to direct exposure to the water in which these microorganisms live. This medium is also used for clinical diagnoses.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Greenish-blue |
| Final pH (25 °C) | 8.0 ± 0.2 |
| Amount | 28.1 g in 500 mL |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bromthymol blue | 0.04 |
| Bacteriological agar | 12.5 |
| Bile salts No. 3 | 3 |
| Ferric ammonium citrate | 0.8 |
| Inositol | 2.5 |
| Lactose | 1.5 |
| L-Arginine hydrochloride | 2 |
| L-Lysine hydrochloride | 3.5 |
| Proteose peptone | 5 |
| Sodium chloride | 5 |
| Sodium thiosulfate | 10.67 |
| Sorbitol | 3 |
| Thymol blue | 0.04 |
| Xylose | 3.75 |
| Yeast extract | 3 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 28.1 grams of the medium in 500 mL of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. AVOID OVERHEATING. DO NOT AUTOCLAVE. Cool to 45-50 °C and aseptically add one vial of Ampicillin Supplement (LC-6052). Homogenize gently and dispense into Petri dishes.
Membrane filtration method
- Filter an appropriate volume of the sample through the membrane.
- Place the membrane on the surface of the agar plate, avoiding the formation of air bubbles.
- Invert the plates and incubate at 30-35 °C for 24 hours.
Quality control
Microbiological test
Incubation conditions: 30-35 °C / 24 h.
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Escherichia coliATCC 25922 | Inhibition | |
| Pseudomonas aeruginosaATCC 27853 | Good growth | Blue-green colonies |
| Aeromonas hydrophilaATCC 7966 | Good growth | Green colonies with a black center |
| Pseudomonas aeruginosaATCC 9027 | Good growth | Blue-green colonies |
Bibliography
- Ryan N. (1985) Personal communication.
- Rogol M., Sechter I., Grinberg L., Gerichter Ch. B. (1992) J. Med. Microbiol. 12. 229-331.
- Atkinson M. (1986) Culture Vol. 7, No. 2.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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