Supplements and additives
Ampicillin Supplement (Aeromonas)
Sterile selective supplement used for Aeromonas spp. isolation from water, food and clinical samples.
- Cat. No.
- LC-6052
- Category
- Supplements and additives
- Format
- 10 vials, each for 500 mL of medium
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Minimum order
- 30 packs
Ampicillin permits the isolation of Aeromonas spp., which are getting increasing attention as human pathogens.
Key features
Applications
Selective isolation of Aeromonas.
Industry
Water.
Presentation
10 freeze-dried vials with 3 ± 0.1 g.
Packaging
23x60 mm glass vials, tag labelled, white plastic cap - 10 vials per box.
Description and technique
Description
The macroscopic aspect of the colonies of Aeromonas may depend on the formulation of the medium base, as follows:
- Dark green, opaque colonies with a dark center in Ryan's medium.
- Brilliant pink translucent colonies in media formulated with crystal violet and neutral red.
- Yellow colonies due to the dextrin fermentation in selective medium following Havelaar.
Technique
Collect and process sample volumes according to the specifications of directives, regulations, standards or specific protocols established depending on the objectives.
Reconstitute the vial with the sterile diluent (6 mL) in aseptic conditions and add it to 500 mL of Blood Agar Base or Columbia Blood Agar Base cooled to 50 °C. Do not overheat once supplemented.
Pour the complete medium into Petri dishes and, once solidified on a flat surface, spread the plates either by streaking or by spiral method.
Incubate the plates in aerobic atmosphere at 35 ± 2 °C for 24-48 h.
Incubation times longer than those mentioned above or different incubation temperatures may be required depending on the sample or the specifications.
After incubation, count all the colonies that have appeared on the surface of the agar.
Presumptive isolation of Aeromonas spp. must be confirmed by further microbiological and biochemical tests.
Presentation
| Presentation | 10 freeze-dried vials |
|---|---|
| Filling | 3 ± 0.1 g |
| Packaging | 23x60 mm glass vials, tag labelled, white plastic cap - 10 vials per box. |
Composition
| Ingredient | g/vial |
|---|---|
| Ampicillin sodium salt | 0.0025 |
Reconstitute the original freeze-dried vial by adding 6 mL of sterile distilled water.
Note: each vial is sufficient to supplement 500 mL of medium Blood Agar Base (Columbia).
Quality control
Physical/chemical control
| Color | White-grey |
|---|
Microbiological control
Reconstitute 1 vial as indicated in Composition; shake and dissolve completely.
Distribute the complete medium, cooled to 50 °C, in plates.
Analytical methodology according to ISO 11133:2014/Amd 1:2018; A2:2020.
Aerobiosis. Incubation at 35 ± 2 °C, reading at 24-48 hours.
| Microorganism | Growth |
|---|---|
| Aeromonas hydrophilaATCC 7966, WDCM 00063 | Good |
| Escherichia coliATCC 25922, WDCM 00013 | Partially inhibited (≤ 30%) |
| Stph. aureusATCC 25923, WDCM 00034 | Inhibited |
Sterility control
Add 5 mL of the sample to 100 mL of TSB and to 100 mL of Thioglycollate.
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Bibliography
- ATLAS, RM & LC PARKS (1993) Handbook of Microbiological Media. CRC Press. London.
- CASMAN, E. (1947) A non-infusion blood agar base for neisseriae, pneumococci and streptococci. Am. J. Clin. Path. 17:281-289.
- ELLNER, PD, CJ STOESSEL, E. DRAKEFORD, & F. VASI (1966) A new culture medium for medical bacteriology. Amer. J. Clin. Path. 45:502-504.
- ISENBERG H.D. (1992) Clinical Microbiology Procedures Handbook. ASM Washington. DC. USA.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.