Supplements and additives
Novobiocin Selective Supplement (10 mg)
Sterile selective supplement used for the isolation of E. coli O157:H7.
- Cat. No.
- LC-6048
- Category
- Supplements and additives
- Format
- 10 vials, each for 500 mL of medium
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Standards
- ISO 16654
Novobiocin is used in different culture media, like TSB Tryptone Soy Broth or Semisolid Rappaport-Vassiliadis Modified, in order to isolate E. coli O157:H7 and/or Salmonella spp., enhancing the inhibition of Gram-positive microorganisms.
Key features
Applications
Selective enumeration and selective isolation of Escherichia coli O157.
Industry
Food.
Standards
ISO 16654.
Presentation
10 freeze-dried vials with 3 ± 0.1 g.
Description and technique
Technique
Collect, dilute and prepare samples and volumes as required according to specifications, directives, official standard regulations and/or expected results.
Reconstitute the vial with a sterile diluent in aseptic conditions and add it to 500 mL of medium base cooled to room temperature.
Do not overheat once supplemented.
Pour the complete medium into suitable containers.
Inoculate and incubate according to normatives and specifications.
Presumptive isolation of the required microorganism must be confirmed by further tests.
For E. coli O157 enrichment use Tryptone Soya Broth
Following the instructions of ISO 16654:2001, the detection of E. coli O157:H7 is developed in four consecutive steps:
- Enrichment of the sample in TSB + novobiocin.
- Separation and concentration with immunomagnetic techniques.
- Isolation in two different agars: the first one CT SMAC/MacConkey Sorbitol and a second one freely chosen by the user.
- Confirmation of the presumptive colonies of E. coli O157:H7 with biochemical tests.
Reconstitute the vial with sterile distilled water, pre-warmed to approx. 37 °C, and add to 500 mL of sterilized broth base cooled to room temperature.
Incubate the tubes tightly closed in aerobic atmosphere at 42 ± 2 °C for 24 h.
Follow the instructions detailed in the ISO standard.
Presentation
| Presentation | 10 freeze-dried vials |
|---|---|
| Filling | 3 ± 0.1 g |
| Packaging | 22 ± 0.25 x 55 ± 0.5 mm glass vials, tag labelled, white plastic cap - 10 vials per box. |
Composition
| Ingredient | g/vial |
|---|---|
| Novobiocin | 0.010 |
Reconstitute the original freeze-dried vial by adding 6 mL of sterile distilled water.
Note: each vial is sufficient to supplement 500 mL of medium base: TSB Tryptone Soy Broth or Semisolid Rappaport-Vassiliadis Modified.
Quality control
Physical/chemical control
| Color | White-grey |
|---|
Microbiological control
Reconstitute 1 vial as indicated in Composition; shake and dissolve completely.
Add 1 vial to 500 mL of medium base. DO NOT HEAT once supplemented.
Distribute the complete medium, cooled to 50 °C, into 10 mL tubes.
Incubate according to the instructions for the complete medium indicated in Composition.
Anaerobiosis. Incubation at 44 ± 1 °C for 21 ± 3 h.
| Microorganism | Growth |
|---|---|
| E. coli O157:H7 (non-toxigenic)ATCC 700728, WDCM 00014 | Good |
| Stph. aureusATCC 25923, WDCM 00034 | Inhibited |
Sterility control
Add 5 mL of the sample to 100 mL of TSB and to 100 mL of Thioglycollate.
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Bibliography
- ISO 16654:2001 Microbiology of food and animal feeding stuffs. Horizontal method for the detection of Escherichia coli O157.
- De Smedt, J.M. et al. (1991) Int. J. Food Micro. 13:301-308.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
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