Supplements and additives
Chloramphenicol Selective Supplement (25 mg)
Sterile selective supplement used for yeast and mould isolation according to ISO and Eur. Pharm.
- Cat. No.
- LC-6049
- Category
- Supplements and additives
- Format
- 10 vials, each for 500 mL of medium
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Standards
- European PharmacopoeiaISO 11133
Chloramphenicol selective supplement is added to Sabouraud Agar (or PDA, Malt Extract Agar) in order to obtain a complete medium suitable for the cultivation and differentiation of fungi.
Key features
Applications
Selective enumeration and selective isolation of yeasts and molds.
Standards
European Pharmacopoeia, ISO 11133.
Presentation
10 freeze-dried vials with 3 ± 0.1 g.
Packaging
22 ± 0.25 x 55 ± 0.5 mm glass vials, tag labelled, white plastic cap - 10 vials per box.
Description and technique
Technique
Collect, dilute and prepare samples and volumes as required according to specifications, directives, official standard regulations and/or expected results.
Reconstitute the vial with 6 mL of sterile distilled water in aseptic conditions and add it to 500 mL of sterilized Sabouraud Agar Base cooled to room temperature.
Do not overheat once supplemented.
Once solidified on a flat surface, spread the plates by streaking methodology or by spiral method.
Incubate the plates right side up in aerobic atmosphere at 20-25 °C for 48 h to 5 days.
Incubation times longer than those mentioned above or different incubation temperatures may be required depending on the sample, on the specifications.
After incubation, enumerate all the colonies that have appeared on the surface of the agar.
Presumptive isolation of any pathogenic yeast and/or mould must be confirmed by further microbiological and biochemical tests.
Presentation
| Presentation | 10 freeze-dried vials |
|---|---|
| Filling | 3 ± 0.1 g |
| Packaging | 22 ± 0.25 x 55 ± 0.5 mm glass vials, tag labelled, white plastic cap - 10 vials per box. |
Composition
| Ingredient | g/vial |
|---|---|
| Chloramphenicol | 0.025 |
Reconstitute the original freeze-dried vial by adding 6 mL of sterile distilled water.
Note: each vial is sufficient to supplement 500 mL of medium base.
Quality control
Physical/chemical control
| Color | White-grey |
|---|
Microbiological control
Reconstitute 1 vial as indicated in Composition; shake and dissolve completely.
Add 1 vial to 500 mL of medium base. DO NOT HEAT once supplemented.
Aerobiosis. Incubation at 30-35 °C. Read after 18-24 h to 72 h for bacteria and 3-5 days for fungi.
| Microorganism | Growth |
|---|---|
| Aspergillus brasiliensisATCC 16404 | Good |
| Candida albicansATCC 10231, WDCM 00054 | Good |
| Staphylococcus aureusATCC 6538, WDCM 00032 | Inhibited |
| Escherichia coliATCC 25922, WDCM 00013 | Inhibited |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- AJELLO, L. (1957) Cultural Methods for Human Pathogenic Fungi. J. Chron. Dis. 5:545-551.
- COLIPA (1997) Guidelines on Microbial Quality Management (MQM). Brussels.
- EUROPEAN PHARMACOPOEIA 8.0 (2014) 8th ed. § 2.6.13. Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. EDQM. Council of Europe. Strasbourg.
- GEORGE, L.K., AJELLO, L. & PAPAGEORGE, C. (1954) Use of Cycloheximide in the Selective Isolation of Fungi Pathogenic to Man. J. Lab. Clin. Med, 44 (422-428).
- HANTSCHKE, D. (1968) Mykosen, 11, (769-778).
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- PAGANO, J. LEVIN, J.D. and TREJO, W. (1957-58) Diagnostic Medium for Differentiation of Species of Candida. Antibiotics Annual, 137-143.
- SABOURAUD, R. (1910) Les Teignes. Masson, Paris.
- USP 33 - NF 28 (2011) <62> Microbiological examination of non-sterile products: Test for specified microorganisms. Harmonised Method. USP Corp. Inc. Rockville. MD. USA.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
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