Agaroses
Agarose LM GQT
Agarose with a high resolution for DNA fragments larger than 1000 bp.
- Cat. No.
- LC-809100
- Category
- Agaroses
- Formats
- 5 formats
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
LM GQT Agarose is a low melting temperature agarose with the highest resolving capacity for large DNA fragments (> 1000 bp), including PCR products. This agarose is GQT (Genetic Quality Tested) certified. This ensures that In-Gel applications can be performed in remelted agarose, avoiding difficult DNA extraction steps.
Key features
Industry
Cloning, culture media for molecular biology, PCR and electrophoresis, proteomics.
Principles and uses
LM GQT Agarose is ideal for digestion by agarase enzymes, which makes it very easy to recover large DNA fragments suitable for cloning or enzymatic processing.
LM Agaroses are classified in three categories, depending on the degree of derivatization. Gelling/melting temperatures and gel strength are the most important differences.
Some important features are:
- Lower gel strength than standard agaroses. Even so, gels can be handled easily.
- Higher clarity (gel transparency) than gels of standard agaroses.
- Great sieving capacity.
Technical specifications
| Ash | ≤ 0.4% |
|---|---|
| Gel strength 1% (g/cm²) | ≥ 250 |
| Gelling temperature 1.5% (°C) | 24-28 °C |
| Melting temperature 1.5% (°C) | ≤ 65.5 °C |
| DNase/RNase activity | None detected |
| EEO | ≤ 0.12 |
| Moisture | ≤ 10% |
| Color | White |
| Appearance | Fine, homogeneous powder |
| DNA binding | None detected |
| Comparative assay of different size DNA fragments | Bands appear sharp and finely resolved |
| Background fluorescence assay in ethidium bromide | Very low gel background after EtBr staining |
| In-Gel restriction and ligation (assayed enzymes: EcoRI, HindIII and T4 DNA ligase) | Passes test |
| Digestion with agarase enzyme and DNA recovery | Passes test |
| Sulphate | ≤ 0.1% |
| Storage | 2 to 25 °C |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.