Agaroses
Agarose LM Sieve
Agarose with a high resolution for DNA fragments smaller than 1000 bp.
- Cat. No.
- LC-809200
- Category
- Agaroses
- Formats
- 5 formats
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Agarose LM Sieve is a low melting temperature agarose with the highest resolving capacity for DNA fragments smaller than 1000 bp, especially PCR products ranging from 200 to 800 bp. This agarose is GQT (Genetic Quality Tested) certified. This ensures that In-Gel applications can be performed in remelted agarose, avoiding difficult DNA extraction steps.
Key features
Industry
Cloning, culture media for molecular biology, PCR and electrophoresis, proteomics.
Principles and uses
Agarose LM Sieve is ideal for digestion by agarase enzymes, making it very easy to recover small DNA fragments suitable for cloning or enzymatic processing.
Agarose LM Sieve can be used at high concentrations, forming gels with excellent clarity and a higher sieving capacity than standard melting agaroses. Due to their high gel strength, Agarose LM Sieve gels are very easy to handle, even at concentrations as low as 2%.
Agarose LM Sieve is used in:
- Electrophoresis of DNA fragments of 1000 bp.
- In-Gel enzymatic processing (digestion, ligation, PCR).
- Preparative electrophoresis.
- Analysis and recovery of small DNA fragments for further applications.
Technical specifications
| Ash | ≤ 0.3% |
|---|---|
| DNase/RNase activity | None detected |
| EEO | ≤ 0.10 |
| Moisture | ≤ 10% |
| Color | White |
| Appearance | Fine, homogeneous powder |
| DNA binding | None detected |
| Comparative assay of different size DNA fragments | Bands appear sharp and finely resolved |
| Background fluorescence assay in ethidium bromide | Very low gel background after EtBr staining |
| In-Gel restriction and ligation (assayed enzymes: EcoRI, HindIII and T4 DNA ligase) | Passes test |
| Digestion with agarase enzyme and DNA recovery | Passes test |
| Gel strength 4% (g/cm²) | ≥ 1000 |
| Gelling temperature 4% (°C) | ≤ 35 °C |
| Melting temperature 4% (°C) | ≤ 65 °C |
| Sulphate | ≤ 0.12% |
| Storage | 2 to 25 °C |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.