Dehydrated culture media
Alkaline Peptone Water ISO
For the pre-enrichment and selective enrichment of Vibrio spp.
- Cat. No.
- LC-2155
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Standards
- ISO 21872
- Also available
- Dehydrated medium, 1 format (LC-1407)
- Also available
- Ready-to-use medium, 2 formats (LC-4018)
Alkaline Saline Peptone Water is used for the pre-enrichment and selective enrichment of Vibrio spp. for the detection of potentially enteropathogenic Vibrio parahaemolyticus, Vibrio cholerae and Vibrio vulnificus.
Key features
Applications
Enrichment of Vibrio.
Industry
Food.
Standards
ISO 21872.
Preparation
40 g in 1 liter of distilled water; boil for 1 minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Vibrio pathogenic to humans causes cholera, choleraic diarrhea or food poisoning from contaminated foods and from stool specimens. The last two conditions especially can be caused by ingesting raw or partially processed fish or seafood containing Vibrio parahaemolyticus.
Peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride promotes growth (Vibrio grows well in salty media).
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Clear amber |
| Final pH (25 °C) | 8.6 ± 0.2 |
| Amount per litre | 40 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Peptone | 20 |
| Sodium chloride | 20 |
Preparation and use
Preparation
Suspend 40 grams of the medium in 1 liter of distilled water. Mix well and dissolve by boiling for 1 minute, stirring constantly. Distribute in suitable containers and sterilize in an autoclave at 121 °C for 15 minutes.
According to ISO 21872
According to ISO 21872 for the detection of potentially enteropathogenic Vibrio spp.:
- Inoculate the test portion in Alkaline Saline Peptone Water and incubate at 41.5 °C for 6 hours and/or 37 °C for 6 hours.
- Inoculate the culture obtained in Alkaline Saline Peptone Water again, and incubate at 41.5 °C for 18 hours and/or 37 °C for 18 hours.
- The incubation conditions depend on the target species or product state.
- From the cultures obtained in the first and the second liquid selective enrichment medium (Alkaline Saline Peptone Water), inoculate two selective media: TCBS Agar (LC-1074) and any other selective medium complementary to TCBS Agar (for instance, Vibrio Chromogenic Agar (LC-2054)).
- Incubate the TCBS plates at 37 °C for 24 hours.
- Incubate the second selective medium in accordance with the manufacturer's instructions.
- Subculture the colonies of presumptive Vibrio and confirm by biochemical means and PCR tests.
Quality control
Microbiological test
Incubation conditions: 37 °C / 6 ± 1 h and 37 °C / 18 ± 1 h.
| Microorganism | Specification |
|---|---|
| Vibrio furnissiiNCTC 11218 | Good growth, turbidity |
| Vibrio choleraeATCC 14034 | Good growth, turbidity |
| Vibrio parahaemolyticusATCC 17802 | Good growth, turbidity |
| Vibrio vulnificusATCC 27562 | Good growth, turbidity |
Bibliography
- APHA AWWA WEF (1998) Standard Methods for the examination of water and wastewater. 20th ed. APHA. Washington, DC.
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media, CRC Press Inc., London.
- DOWNES, F.P. & K. ITO (2001). Compendium of Methods for the Microbiological Examination of Food. 4th Ed. APHA, Washington.
- ISO 21872-1 Technical Specification (2017) Microbiology of Food chain - Horizontal method for the detection of potentially enteropathogenic Vibrio spp. - Part 1: Detection of Vibrio parahaemolyticus and Vibrio cholerae and Vibrio vulnificus.
- ISO 11133:2014. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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