Ready-to-use media
Alkaline Peptone Water ISO
Liquid culture medium for the pre-enrichment and selective enrichment of potentially enteropathogenic vibrios, in food samples, according to ISO.
- Cat. No.
- LC-4018
- Category
- Ready-to-use media
- Formats
- 2 formats
- Minimum order
- 15 packs
- Standards
- ISO 21872
- Also available
- Dehydrated medium, 1 format (LC-1407)
- Also available
- Dehydrated medium, 1 format (LC-2155)
Technical specifications ISO 21872 in parts 1 and 2 describe this liquid culture medium for use in the pre-enrichment and selective enrichment of potentially enteropathogenic vibrios, from food samples.
Key features
Applications
Selective enrichment of Vibrio.
Industry
Food.
Standards
ISO 21872.
Description and technique
For pre-enrichment, the sample diluted in the medium is incubated at 37 °C for 6 ± 1 hours and from this culture the selective enrichment medium is inoculated and incubated at the same temperature for 18 ± 1 hours before proceeding to isolation on selective media.
If the presence of V. parahaemolyticus or V. cholerae is suspected, then the pre-enrichment is carried out in the same manner only if the sample is a frozen food sample. With fresh foods the incubation must be carried out at 41.5 °C, always for 6 ± 1 hours. Similarly the selective enrichment is carried out at 41.5 °C for a period of 18 ± 1 h for these species of Vibrio, before proceeding to a selective medium for isolation.
Presentation
| Code | Presentation | Filling | Packaging |
|---|---|---|---|
| LC-4018 | 20 tubes, 16 x 113 mm | 10 ± 0.2 mL | 1 box with 20 tubes, 16 x 113 mm glass tubes, ink labelled and metal non-injectable cap. |
| LC-4685 | 10 prepared bottles, 250 mL | 225 ± 5 mL | 1 box with 10 bottles 250 mL. Injectable cap: plastic screw inner cap + protective outer blue cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
Composition
| Ingredient | g/L |
|---|---|
| Peptone | 20.00 |
| Sodium chloride | 20.00 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 8.6 ± 0.2 at 25 °C |
Microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity).
Keep incubation for 6 ± 1 h.
Subculture after incubation onto TCBS.
| Microorganism | Growth |
|---|---|
| Vibrio alginolyticusATCC 17749 | Good - opaque yellowish colonies |
| Vibrio parahaemolyticusATCC 17802, WDCM 00037 | Good - green colonies |
| Vibrio furnissiiNCTC 11218, WDCM 00186 | Good - opaque yellowish colonies |
LC-4685, bottles: microbiological control
Inoculate: practical range 100 ± 20 CFU; min. 50 CFU (productivity) / 10⁴-10⁶ (selectivity).
Keep incubation for 6 ± 1 h.
Subculture after incubation onto TCBS.
| Microorganism | Growth |
|---|---|
| Vibrio alginolyticusATCC 17749 | Good - opaque yellowish colonies |
| Vibrio parahaemolyticusATCC 17802, WDCM 00037 | Good - green colonies |
| Vibrio furnissiiNCTC 11218, WDCM 00186 | Good - opaque yellowish colonies |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- APHA AWWA WEF (1998) Standard Methods for the examination of water and wastewater. 20th ed. APHA. Washington, DC.
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media, CRC Press Inc., London.
- DOWNES, F.P. & K. ITO (2001). Compendium of Methods for the Microbiological Examination of Food. 4th Ed. APHA, Washington.
- ISO 21872-1 Technical Specification (2017) Microbiology of Food chain - Horizontal method for the detection of potentially enteropathogenic Vibrio spp. - Part 1: Detection of Vibrio parahaemolyticus and Vibrio cholerae and Vibrio vulnificus.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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