PCR and qPCR mixes
AQ97 HiFi 2x Master Mix
High-fidelity proofreading 2x master mix (>60x Taq) for cloning, NGS and long amplicons
- Cat. No.
- A770201
- Category
- PCR and qPCR mixes
- Formats
- 4 formats
- Manufacturer
A ready-to-use 2x master mix containing AQ97 High Fidelity DNA Polymerase, optimised buffer, dNTPs and MgCl2: only primers, template and water are added. The chimeric proofreading polymerase gives more than 60x Taq fidelity and elongates at about 10 seconds per kb. It delivers low-bias amplification of low- to high-GC targets and amplicons up to 11 kb from genomic DNA.
Key features
All in one
Complete 2x master mix: only primers, template and water are added.
Over 60x Taq fidelity
3'-5' proofreading and fast elongation of about 10 s/kb.
Broad GC coverage
Good coverage of templates with low to high GC content.
Long range
Amplicons up to 11 kb from genomic DNA; for longer targets use the stand-alone enzyme, up to 18 kb.
Applications
- Cloning and subcloning
- NGS applications
- SNP analysis
- Mutagenesis
- Long-range PCR
- Amplification of GC-rich targets
Technical details
| Composition | AQ97 High Fidelity DNA Polymerase, optimised buffer, dNTPs, MgCl2 |
|---|---|
| MgCl2 | 1.5 mM in the reaction (adjustable up to 4.5 mM with 25 mM MgCl2) |
| Activity | 5'-3' polymerase and 3'-5' exonuclease (proofreading) |
| Fidelity | More than 60x Taq DNA polymerase |
| Elongation rate | About 10 s/kb |
| Amplicon length | Up to 11 kb from genomic DNA |
| Reaction volume | 50 µL |
| Recommended additives | Betaine Enhancer Solution for GC-rich, long or highly structured targets |
| Storage | Room temperature up to 5 days; 4 °C up to 6 months; -20 °C long term |
| Shipping | On dry ice |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.