PCR and qPCR mixes
AQ97 HiFi Hot Start 2x Master Mix RED
Hot start high-fidelity 2x master mix with red dye for direct gel loading
- Cat. No.
- A810801
- Category
- PCR and qPCR mixes
- Formats
- 4 formats
- Manufacturer
A ready-to-use 2x master mix containing AQ97 Hot Start High Fidelity DNA Polymerase, optimised buffer, dNTPs, MgCl2 and an inert red dye: only primers, template and water are added. Antibody-mediated hot start keeps polymerase and exonuclease inactive during setup, which can be done at room temperature with higher specificity. The dye lets products be loaded straight onto agarose gels without loading buffer. It delivers low-bias amplification of low- to high-GC targets and amplicons up to 11 kb from genomic DNA; the mix stays liquid at -20 °C and is ready after simple mixing.
Key features
All in one
Complete 2x master mix: only primers, template and water are added.
Antibody hot start
Room temperature setup without loss of specificity.
Direct gel loading
The inert red dye runs at 1000-2000 bp on 0.5-1.5% agarose.
Long range
Amplicons up to 11 kb from genomic DNA; for longer targets use the stand-alone enzyme, up to 18 kb.
Applications
- Cloning and subcloning
- NGS applications
- SNP analysis
- Mutagenesis
- Long-range PCR
- Amplification of GC-rich targets
Technical details
| Composition | AQ97 Hot Start High Fidelity DNA Polymerase, optimised buffer, dNTPs, MgCl2, inert red dye |
|---|---|
| Activity | 5'-3' polymerase and 3'-5' exonuclease (proofreading) |
| Hot start | Antibody mediated; initial denaturation 30 s-2 min at 98 °C |
| Amplicon length | Up to 11 kb from genomic DNA |
| Reaction volume | 50 µL |
| Dye migration | 1000-2000 bp on 0.5-1.5% agarose |
| Recommended additives | Betaine Enhancer Solution for GC-rich, long or highly structured targets |
| Storage | Room temperature up to 5 days; 4 °C up to 6 months; -20 °C long term (does not freeze) |
| Shipping | On dry ice |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.