DNA polymerases
AQ97 Hot Start High Fidelity DNA Polymerase
Hot start high-fidelity proofreading DNA polymerase (>60x Taq) for amplicons up to 18 kb
- Cat. No.
- A787501
- Category
- DNA polymerases
- Formats
- 4 formats
- Manufacturer
A thermostable chimeric DNA polymerase with proofreading activity, designed for low-bias, high-fidelity amplification. Antibody-mediated hot start blocks both the polymerase and the exonuclease activity until the initial 2-minute denaturation at 98 °C, so reactions can be set up at room temperature with higher specificity. Fusion to a DNA-binding domain increases speed and processivity: the enzyme extends at about 10 seconds per kb and amplifies targets up to 18 kb from genomic DNA and 25 kb from lambda DNA. Fidelity, measured by an NGS-based method, is more than 60 times that of Taq DNA polymerase.
Key features
Over 60x Taq fidelity
3'-5' proofreading exonuclease activity and a low error rate measured by NGS analysis.
Antibody hot start
Polymerase and exonuclease stay inactive during room temperature setup; activation in 2 minutes.
Long range
Amplicons up to 18 kb from genomic DNA and 25 kb from lambda DNA.
Broad GC coverage
Amplifies AT-rich to GC-rich targets; tested on human genomic DNA from 29% to 78% GC.
Applications
- Cloning and subcloning
- NGS applications
- SNP analysis
- Mutagenesis
- Long-range PCR
- Amplification of GC-rich or AT-rich targets
Technical details
| Concentration | 2 U/µL |
|---|---|
| Enzyme type | Thermostable chimeric DNA polymerase with DNA-binding domain, antibody-mediated hot start |
| Activity | 5'-3' polymerase and 3'-5' exonuclease (proofreading) |
| Fidelity | More than 60x Taq DNA polymerase (NGS analysis) |
| Elongation rate | About 10 s/kb |
| Amplicon length | Up to 18 kb (genomic DNA), 25 kb (lambda DNA) |
| Activation | 2 minutes at 98 °C |
| Supplied with | 5x AQ97 Buffer (7.5 mM MgCl2, 1.5 mM in the reaction) and 25 mM MgCl2 |
| Storage buffer | 50 mM Tris-HCl pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1.0 mM DTT, 0.1% polysorbate 20, 50% glycerol |
| Recommended additives | Betaine Enhancer Solution 1-2 M for GC-rich, long or highly structured targets |
| Storage | Room temperature up to 5 days; 4 °C up to 6 months; -20 °C long term |
| Shipping | On dry ice |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.