Dehydrated culture media
BCP Agar
For the isolation of coliforms.
- Cat. No.
- LC-1051
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
BCP Agar is a non-inhibitory medium used for the detection and isolation of coliforms and in differential studies based on lactose fermentation. BCP stands for bromocresol purple.
Key features
Applications
Selective isolation of coliforms.
Industry
General cultivation.
Preparation
28 g in one liter of distilled water; boil for one minute, sterilize at 121 °C for 15 minutes.
Principles and uses
Peptone mixture and beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Lactose is the fermentable carbohydrate providing carbon and energy. Bromocresol purple is a pH indicator. Bacteriological agar is the solidifying agent.
All coliforms ferment lactose with acid and gas production. This group includes the genera Escherichia, Enterobacter, Citrobacter, and Klebsiella. When lactose is fermented it produces acid that changes the color of the medium from blue-purple (alkaline) to yellow (acid). Blue colonies are lactose-negative and yellow colonies are lactose-positive.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Purple |
| Final pH (25 °C) | 6.8 ± 0.2 |
| Amount per litre | 28 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 10 |
| Beef extract | 3 |
| Bromocresol purple | 0.025 |
| Lactose | 10 |
| Peptone mixture | 5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 28 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize at 121 °C for 15 minutes. Cool to 45-50 °C, mix well and dispense into plates.
Instructions for use
- Inoculate and incubate at 35 ± 2 °C.
- Reading must be carried out after 18-24 hours as longer incubation times may cause the diffusion of the acid in the medium and result in an error.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Klebsiella pneumoniaeATCC 13883 | Good growth | Colony color: yellow |
| Salmonella typhimuriumATCC 14028 | Good growth | Colony color: blue |
| Escherichia coliATCC 25922 | Good growth | Colony color: yellow |
| Shigella sonneiATCC 25931 | Good growth | Colony color: blue |
Bibliography
- Finegold, S.M., E.J. Baron 1986 Bailey and Scott's Diagnostic Microbiology 7th ed. C.V. Mosby, St. Louis
- Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American Society for Microbiology.
- Mac Faddin, Jean F., Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria Vol.1 1985 Baltimore, MD. Williams & Wilkins
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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