Dehydrated culture media
Tryptose Agar
For the cultivation of a wide variety of fastidious microorganisms, particularly Brucella.
- Cat. No.
- LC-1047
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Tryptose Dextrose Agar with thiamine is a general purpose non selective medium recommended for the cultivation of Brucella spp.
Key features
Applications
Growth of Brucella and fastidious microorganisms.
Preparation
41 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Sanders and Huddleson demonstrated that the addition of dextrose and thiamine chlorohydrate to the medium stimulates the growth of some species of Brucella. The Tryptose Dextrose Agar with thiamine is also recommended for the cultivation of pathogen microorganisms without enrichment, for streptococci, pneumococci, meningococci and other fastidious bacteria.
Tryptose provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dextrose is the fermentable carbohydrate providing carbon and energy. Thiamine is a growth factor. Bacteriological agar is the solidifying agent.
The high productivity of the Tryptose Dextrose Agar used for the cultivation and isolation of Brucella confirms its value for the primary cultivation of Brucella, as other fastidious organisms.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.2 ± 0.2 |
| Amount per litre | 41 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 1 |
| Bacteriological agar | 15 |
| Sodium chloride | 5 |
| Thiamine hydrochloride | 0.005 |
| Tryptose | 20 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 41 grams of medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C, mix well and dispense into plates.
Instructions for use
Inoculate and incubate at 35 ± 2 °C under 5-10% of CO₂ for 40-48 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 40-48 h.
The microbiological test of the Brucella species should be carried out by the end-user laboratory.
| Microorganism | Specification |
|---|---|
| Streptococcus pyogenesATCC 19615 | Good growth |
| Streptococcus pneumoniaeATCC 6305 | Good growth |
Bibliography
- Jones, L.M., Wundt, W.; International Committee on Nomenclature of Bacteria, Subcommittee on the Taxonomy of Brucella. - Int. J. Syst. Bact., 21; 126-128 (1971).
- Picket, M.J., Nelson, E.L., a. Liberman, J.D.; Specification within the Genus Brucella. II. Evaluation of Differential Dye, Biochemical, and Serological Tests. - J. Bact, 66; 210-219 (1953).
- Schindler, R.: Untersuchungen über die Differenzierung von Brucellatypen. - Zbl. Bakt., I. Orig., 164; 93-95 (1955).
- Silverman, S.J., a. Elberg, S.S.; The antigenic relationships of native antigens of species of Brucella. - J. Immunol., 65; 163-174 (1950).
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