Dehydrated culture media
BCP Glucose Broth
For the differentiation of Enterobacteriaceae.
- Cat. No.
- LC-2009
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
BCP Glucose Broth is used for the differentiation of Enterobacteriaceae in water and food. It differentiates species on the basis of dextrose fermentation.
Key features
Applications
Differentiation of enterobacteria.
Industry
Food, water.
Preparation
26.5 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Tryptone and yeast extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Glucose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bromocresol purple is a pH indicator. Bacteriological agar is the solidifying agent.
Inoculate and incubate at 35 ± 2 °C for 18-24 hours. The glucose-fermenting microorganisms produce a change of the color of the medium to yellow colonies (acid) and the medium will maintain the purple color with the non-fermenting bacteria.
This medium can be used for glucose fermentation testing. Inoculate oxidase-negative colonies in tubes containing BCP Glucose Broth and incubate. A yellow color indicates a positive reaction. Oxidase-negative and glucose-positive microorganisms are confirmed as Enterobacteriaceae.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Purple |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 26.5 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Glucose | 10 |
| Bromocresol purple | 0.015 |
| Sodium chloride | 5 |
| Tryptone | 10 |
| Yeast extract | 1.5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 26.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Inoculate and incubate at a temperature of 35 ± 2 °C for 18-24 h.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Salmonella typhimuriumATCC 14028 | Good growth | Acid production (color changes to yellow) |
| Escherichia coliATCC 25922 | Good growth | Acid production (color changes to yellow) |
| Staphylococcus aureusATCC 25923 | Good growth | No acid production |
Bibliography
- ISO 21528-2 Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 2: Colony-count method.
- Drigalsky, C. (1902) Uber ein Verfahren zum Nachweis der Typhusbacillen.
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