Dehydrated culture media
Yeast Mold Broth
For the cultivation of yeast, molds and other aciduric microorganisms.
- Cat. No.
- LC-2008
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Yeast Mold Broth is a medium used for the isolation and cultivation of yeast, molds and aciduric microorganisms.
Key features
Applications
Growth of yeasts and molds.
Industry
Food.
Preparation
20 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Peptone and malt extract provide the carbon, protein and nutrient sources required for the growth of microorganisms.
Malt extract is particularly suitable for yeasts and molds as it contains a high concentration of maltose (39-42%) and other saccharides as energy sources. Dextrose is the fermentable carbohydrate providing carbon and energy. The high dextrose concentration and acidic pH make this medium selective for fungi.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Light beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 6.2 ± 0.2 |
| Amount per litre | 20 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 10 |
| Malt extract | 3 |
| Peptone | 4 |
| Yeast extract | 3 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 20 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Distribute into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
If desired, the pH of the medium can be adjusted to 3.0-4.0 in order to increase the selectivity of the medium. Antibiotics like chloramphenicol can also be added.
Instructions for use
Inoculate and incubate at 30 ± 2 °C for 18-72 hours.
Quality control
Microbiological test
Incubation conditions: 30 ± 2 °C / 18-72 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Aspergillus brasiliensisATCC 16404 | Good growth |
| Saccharomyces cerevisiaeATCC 9763 | Good growth |
Bibliography
- Jong, S.S. and M.J. Edwards. 1991. American Type Culture Collection Catalog of filamentous fungi, 18th ed. American Type Collection, Rockville, MD.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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