Ready-to-use media
Brilliant Green Bile Broth 2% (Doble Concentration) ISO
Liquid medium for the detection of coliforms in water according to ISO and APHA standards.
- Cat. No.
- LC-4253
- Category
- Ready-to-use media
- Format
- 20 tubes
- Storage
- 8 to 25 °C
- Minimum guaranteed shelf life
- 4 months
- Standards
- ISO 4831ISO 4832
Brilliant Green Bile 2% Broth has been widely used as a medium for the assay of presumptive colimetries in food, milk and water, through the Most Probable Number Technique. This broth offers some advantages over other similar broths as its balanced composition of Bile and Brilliant Green effectively suppresses the growth of Gram-positive bacteria, even that of the more fastidious Clostridium perfringens.
Key features
Applications
Confirmation of coliforms.
Industry
Food, water.
Standards
ISO 4831, ISO 4832.
Presentation
20 tubes, 17 x 145 mm, with 10 ± 0.2 mL.
Description and technique
Description
It is recommended by the APHA for colimetries in water, milk and food. British and Australian methodology use the broth as an intermediate stage between presumptive and confirmative colimetry, as if it were an enrichment at 30 ± 1 °C. Other authors suggest it as an optimal base for the Eijkman testing of gas production at 44 ± 1 °C, for the identification of E. coli.
Technique
To inoculate tubes, follow the standard laboratory methods or the applicable norms: stab inoculation, loop inoculation, dilution banks, Most Probable Number (MPN) technique, pipette inoculation, etc.
Presentation
| Presentation | 20 tubes, 17 x 145 mm |
|---|---|
| Filling | 10 ± 0.2 mL |
| Packaging | 17 x 145 mm glass tubes, ink labelled, metal cap + Durham tube. 20 tubes per box. |
Composition
| Ingredient | g/L |
|---|---|
| Peptone | 20.0000 |
| Bile | 40.0000 |
| Lactose | 20.0000 |
| Brilliant green | 0.0266 |
Quality control
Physical/chemical control
| Color | Green |
|---|---|
| pH | 7.2 ± 0.3 at 25 °C |
Microbiological control
Inoculate: practical range 10-100 CFU (productivity) / 10⁴-10⁶ (selectivity).
Dilute to normal concentration.
Aerobiosis. Incubation at 30 ± 1 °C, reading after 24-48 h.
| Microorganism | Growth |
|---|---|
| Escherichia coliATCC 25922, WDCM 00013 | Good - gas positive |
| Salmonella typhimuriumATCC 14028, WDCM 00031 | Good - gas negative |
| Enterococcus faecalisATCC 29212, WDCM 00087 | Partial inhibition |
| Citrobacter freundiiATCC 43864, WDCM 00006 | Good - gas positive |
| Escherichia coliATCC 8739, WDCM 00012 | Good - gas positive |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- APHA (1971) Standard Methods for the Examination of Water and Wastewater. 13th ed. Washington.
- DOWNES, F.P. & K. ITO (2001) Compendium of Methods for the Microbiological Examination of Foods. 4th ed. APHA. Washington.
- FDA (Food and Drug Administration) (1998) Bacteriological Analytical Manual. 8th ed. Rev. A. AOAC Intl. Gaithersburg. MD. USA.
- ISO 4831 Standard (2006) Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of coliforms - MPN Technique.
- ISO 9308-1 Standard (1990) Water quality. Detection and enumeration of coliforms, thermotolerant coliforms and E. coli. MPN Method.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- PASCUAL ANDERSON, Mª R. (1992) Microbiología Alimentaria. Díaz de Santos. S.A. Madrid.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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Frequently asked questions
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