Ready-to-use media
Buffered Peptone Water ISO
Dilution and non-selective pre-enrichment liquid medium according to ISO standards.
- Cat. No.
- LC-4250
- Category
- Ready-to-use media
- Formats
- 7 formats
- ISO 17025 quality control
- Yes
- Storage
- 8 to 25 °C
- Standards
- ISO 22964ISO 6579ISO 6887
- Also available
- Dehydrated medium, 1 format (LC-1401)
- Also available
- Dehydrated medium, 1 format (LC-1402)
- Also available
- Ready-to-use medium, 2 formats (LC-4643)
This formulation of Buffered Peptone Water has the advantages of the two classical diluents used for food samples: it has the property of revitalization of the peptone water and the pH change absorbing capacity of the phosphate buffer.
Key features
Applications
Diluent and enrichment (general use).
Industry
Cosmetics, food, pharmaceutical/veterinary.
Standards
ISO 22964, ISO 6579, ISO 6887.
Testing
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Description and technique
Description
The composition of this diluent is made according to the specification of the ISO Standard 6579 for the detection of Salmonella in foods and other ISO Standards (6785, 6887 and 8261).
Technique
Inoculate according to final purpose, samples and validated methods.
LC-5020 and LC-5171, bottles
Diluent and non-selective pre-enrichment broth for the homogenization of food samples formulated according to ISO 6579, ISO 8523 and ISO 6785.
Use the medium according to intended purposes, samples and validated methods.
LC-6702, LC-6705 and LC-6707, bags
Each bag is intended for use with an automatic dispenser in laboratories requiring large volumes of broth media or diluent.
Discard any partially used bag to avoid contamination.
The bag has multiple connection points: 1 penetrable cap (injection port) of latex-free polycarbonate, for any additive injection required, and an injection (vial stopper) to connect to any standard laboratory dosing equipment with a connector.
Once completely empty, the bag can be disposed of along with normal plastic (PVC).
Presentation
| Code | Presentation | Filling | Packaging |
|---|---|---|---|
| LC-4250 | 20 tubes, 16 x 113 mm | 9 ± 0.1 mL | 1 box with 20 tubes, 16 x 113 mm glass tubes, ink labelled and metallic cap. |
| LC-5020 | 10 prepared bottles, 250 mL total capacity | 225 ± 5 mL | 1 box with 10 bottles 250 mL. Injectable cap: plastic screw inner cap + protective outer blue cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
| LC-5153 | 10 prepared bottles, 125 mL | 100 ± 3 mL | 1 box with 10 bottles 125 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
| LC-5171 | 10 prepared bottles, 125 mL total capacity | 90 ± 3 mL | 1 box with 10 bottles 125 mL. Injectable cap: plastic screw inner cap. The use of syringe needles with a diameter greater than 0.8 mm is not recommended. |
| LC-6702 | 2 prepared bags, 5 L | 5000 ± 15 mL | 1 box with 2 bags of 5 L. PVC plasticizer free sterile bag with 1 vial stopper + 1 penetrable cap. Dimensions: 27 x 40 cm. For use in food testing. |
| LC-6705 | 3 prepared bags, 3 L | 3000 ± 10 mL | 1 box with 3 bags of 3 L. PVC plasticizer free sterile bag with 1 vial stopper + 1 penetrable cap. Dimensions: 23 x 32 cm. For use in food testing. |
| LC-6707 | 5 prepared bags, 2 L | 2000 ± 15 mL | 1 box with 5 bags of 2 L. PVC plasticizer free sterile bag with 1 vial stopper + 1 penetrable cap. Dimensions: 18 x 32 cm. For use in food testing. |
Composition
| Ingredient | g/L |
|---|---|
| Casein peptone | 10.0 |
| Sodium chloride | 5.0 |
| Disodium phosphate (anhydrous) (1) | 3.5 |
| Potassium phosphate | 1.5 |
(1) Equivalent to 9.0 g of disodium hydrogen phosphate dodecahydrate.
LC-5020, 250 mL bottles
| Ingredient | g/L |
|---|---|
| Tryptone | 10.00 |
| Sodium chloride | 5.00 |
| Disodium phosphate | 9.00 |
| Potassium phosphate | 1.50 |
LC-5153 and LC-5171, 125 mL bottles
| Ingredient | g/L |
|---|---|
| Casein peptone | 10.0 |
| Sodium chloride | 5.0 |
| Disodium phosphate 12 H₂O | 9.0 |
| Potassium phosphate | 1.5 |
Quality control
Physical/chemical control
| Color | Yellow |
|---|---|
| pH | 7.0 ± 0.2 at 25 °C |
Microbiological control
Prepare tubes / inoculate ≤ 10³ CFU/tube (productivity) / subculture after holding at 20-25 °C for 45 min to 1 h.
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobiosis. Incubation at 35 ± 2 °C. Reading at 24 hours.
| Microorganism | Growth |
|---|---|
| Stph. aureusATCC 25923, WDCM 00034 | Good. Recovery ± 30% T0 (original enumeration) |
| Escherichia coliATCC 25922, WDCM 00013 | Good. Recovery ± 30% T0 (original enumeration) |
| Escherichia coliATCC 8739, WDCM 00012 | Good |
| Salmonella typhimuriumATCC 14028, WDCM 00031 | Good |
| Salmonella entericaATCC 13076, WDCM 00030 | Good |
LC-5020: microbiological control
Prepare tubes / inoculate 10³-10⁴ (productivity) / subculture after holding at 20-25 °C for 45 min to 1 h.
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobiosis. Incubation at 36 ± 2 °C, reading at 18 ± 2 h.
| Microorganism | Growth |
|---|---|
| Stph. aureusATCC 25923, WDCM 00034 | Good. Recovery ± 30% T0 (original enumeration) |
| Escherichia coliATCC 25922, WDCM 00013 | Good. Recovery ± 30% T0 (original enumeration) |
LC-5153, LC-5171, LC-6702, LC-6705 and LC-6707: microbiological control
Prepare tubes / inoculate 100 ± 20 CFU (productivity) / subculture after holding at 20-25 °C for 45 min to 1 h, E. coli & Staphyl.; Listeria 1 h at 20 ± 2 °C.
Microbiological control according to ISO 11133:2014/Amd 1:2018.
Aerobiosis. Incubation at 37 ± 1 °C, reading after 18 ± 2 h.
| Microorganism | Growth |
|---|---|
| Stph. aureusATCC 25923, WDCM 00034 | Good. Recovery ± 30% T0 (original enumeration) |
| Escherichia coliATCC 25922, WDCM 00013 | Good. Recovery ± 30% T0 (original enumeration) |
| Escherichia coliATCC 8739, WDCM 00012 | Good |
| Salmonella typhimuriumATCC 14028, WDCM 00031 | Good |
| Salmonella entericaATCC 13076, WDCM 00030 | Good |
| L. monocytogenesATCC 13932, WDCM 00021 | Good. Recovery ± 30% T0 (original enumeration) |
| Listeria monocytogenesATCC 35152 | Good. Recovery ± 30% T0 (original enumeration) |
| Escherichia coliATCC 8739, WDCM 00012 (LC-5153, LC-5171 and LC-6702) | Good. Recovery ± 30% T0 (original enumeration) |
Sterility control
Incubation 48 hours at 30-35 °C and 48 hours at 20-25 °C: NO GROWTH.
Check at 7 days after incubation in same conditions.
Bibliography
- ATLAS, R.M. & L.C. PARKS (1993) Handbook of Microbiological Media. CRC Press, Inc. London.
- ISO 11133:2014/Amd 1:2018. Microbiology of food, animal feed and water. Preparation, production, storage and performance testing of culture media.
- ISO Standard 6579-1 (2017) Microbiology of food chain - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 1: Detection of Salmonella spp.
- ISO 6785 (2001) Milk and milk products. Detection of Salmonella spp.
- ISO 6887-1 (1999) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 1: General rules for the preparation of the initial suspension and decimal dilutions.
- ISO 6887-2 (2003) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 2: Specific rules for the preparation of meat and meat products.
- ISO 6887-3 (2003) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 3: Specific rules for the preparation of fish and fishery products.
- ISO 6887-4 (2003) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 4: Specific rules for the preparation of products other than milk and milk products, meat and meat products and fish and fishery products.
- ISO/DIS 6887-5 (2009) Microbiology of food and animal feeding stuffs - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination. Part 5: Specific rules for the preparation of milk and milk products.
- ISO 8261 (2001) Milk and milk products. General guidance for the preparation of test samples for microbiological examination.
- ISO 21528-1:2004 Standard. Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1: Detection and enumeration by MPN technique with pre-enrichment.
- ISO/TS 22964 (2006) Milk and milk products - Detection of Enterobacter sakazakii.
- PASCUAL ANDERSON, Mª R. (1992) Microbiología Alimentaria. Díaz de Santos, S.A. Madrid.
- UNE-EN ISO 11133 (2014). Microbiología de los alimentos para consumo humano, alimentación animal y agua - Preparación, producción, conservación y ensayos de rendimiento de los medios de cultivo.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.