Dehydrated culture media
Enterococcus Selective Agar (Enterococcosel Agar)
Selective medium for the enrichment and isolation of enterococci from diverse clinical materials and of highly contaminated products of sanitary importance.
- Cat. No.
- LC-1070
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Enterococcus Selective Agar (Enterococcosel Agar) is basically the same as Enterococcosel Broth (LC-1204) to which 1.2% agar has been added.
Key features
Applications
Selective isolation of enterococci.
Industry
Food, water.
Preparation
42.6 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 118 °C for 15 minutes, do not overheat.
Principles and uses
It is a sensitive enrichment medium for the isolation of streptococci from specimens containing numerous other flora. Many organisms such as saprophytic Neisseria, Staphylococcus, Haemophilus, non-hemolytic streptococci, and a certain number of Enterobacteriaceae are inhibited wholly or partially, permitting satisfactory fluorescence studies of Group A streptococci in 18-24 hours.
Casein and soy peptones provide essential nutrients for growth. Dextrose is the fermentable carbohydrate energy source. Sodium chloride maintains the osmotic balance. Sodium citrate provides additional carbon. Sodium azide is an inhibitor. Sodium sulfite when reduced produces H₂S. L-Cystine lowers the oxidation-reduction potential by removing oxygen to maintain a low Eh. Crystal violet is a pH indicator. Bacteriological agar is the solidifying agent.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber slightly opalescent, with a violet hue |
| Final pH (25 °C) | 7.4 ± 0.2 |
| Amount per litre | 42.6 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 12 |
| Casein peptone | 15 |
| Crystal violet | 0.0002 |
| Dextrose | 5 |
| L-Cystine | 0.2 |
| Sodium azide | 0.2 |
| Sodium chloride | 4 |
| Sodium citrate | 1 |
| Sodium sulfite | 0.2 |
| Soy peptone | 5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 42.6 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 118 °C for 15 minutes. DO NOT OVERHEAT. Cool to 45-50 °C, mix well and dispense into Petri dishes.
Instructions for use
- Inoculate and incubate at 35 ± 2 °C for 18-24 hours.
- Adding 0.5% of sterile defibrinated sheep or rabbit blood notably increases its nutritional power and hemolytic studies can be conducted. These conditions yield good results in the isolation and identification of different groups of Streptococcus such as the alpha and beta-hemolytic, and the non-hemolytic.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-24 h.
| Microorganism | Specification |
|---|---|
| Enterococcus faecalisATCC 19433 | Good growth |
| Escherichia coliATCC 25922 | Inhibited growth |
| Enterococcus faeciumATCC 6057 | Good growth |
Bibliography
- Facklam and Carly, 1985, Manual of Clinical Microbiology, Lennette and others (Eds). 4th Ed. ASM, Washington DC.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
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