Extraction and purification
GENAzol RNA Isolation Reagent
Monophasic guanidinium isothiocyanate reagent for isolating RNA, DNA and protein from the same sample
- Cat. No.
- S5318
- Category
- Extraction and purification
- Formats
- 1 format
- Manufacturer
GENAzol is a ready-to-use monophasic reagent based on an aqueous guanidinium isothiocyanate solution, for isolating total RNA from tissues, cells and other biological material. After chloroform is added, the homogenate separates into three phases: RNA stays in the aqueous phase and is precipitated with isopropanol, while DNA and protein are recovered from the interphase and organic phase. The complete isolation usually takes about one hour.
Key features
Three fractions from one sample
RNA, DNA and protein are obtained from the same homogenate.
Quality RNA
A260/A280 ratio of 1.7 to 1.9, with RNase activity effectively inhibited.
Many sample types
Suitable for human, animal, plant, yeast, bacterial, viral and blood samples.
Fast protocol
Complete isolation usually within one hour.
Applications
- RT-PCR
- Northern blotting
- Dot blot hybridisation
- RNase protection assays
- In vitro translation
- DNA for PCR and Southern blotting
- Protein for Western blotting
Technical details
| Composition | Monophasic reagent based on aqueous guanidinium isothiocyanate solution |
|---|---|
| Sample | 1 mL per 100 mg tissue or up to 10^7 cells |
| RNA purity | A260/A280 1.7-1.9 |
| Time | Usually about 1 hour for complete isolation |
| Required but not supplied | Chloroform, isopropanol, ethanol |
| Storage | +2 °C to +8 °C |
| Shipping | Ambient |
| Safety | Hazardous substance (UN2821); GHS05, GHS06, GHS08 pictograms |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.