DNA ladders, loading buffers and gel stains
GenLadder 1 kb (ready-to-use)
Ready-to-use DNA ladder from 250 bp to 10 kb, with reference bands at 1,000 and 3,000 bp
- Cat. No.
- M3328
- Formats
- 2 formats
- Manufacturer
A synthetic marker with 13 DNA fragments from 250 to 10,000 bp, premixed with 6x loading dye and ready to load. The double bands at 1,000 and 3,000 bp are about twice as intense for easy orientation; compared with an equimolar ladder, bands below 1,000 bp are enhanced and bands above 3,000 bp reduced. It is designed for sizing plasmid inserts (250-3,000 bp in 250 bp steps) and digested plasmid vectors (above 3,000 bp in 1-2 kb steps).
Key features
Ready to use
Premixed with loading dye: 5-6 µL per lane.
13 fragments
From 250 bp to 10 kb, with 250 bp spacing in the typical insert range.
Reference bands
Double, brighter bands at 1,000 and 3,000 bp for instant size reading.
Exact sizes
Each band matches its stated size, with no ambiguous bands.
Applications
- Quantitative molecular weight standard for DNA electrophoresis
- Sizing of plasmid inserts
- Checking plasmid vectors after restriction digestion
Technical details
| Range | 250-10,000 bp |
|---|---|
| Bands | 13: 250, 500, 750, 1,000 (double), 1,500, 2,000, 2,500, 3,000 (double), 4,000, 5,000, 6,000, 8,000, 10,000 bp |
| Concentration | About 0.1 µg/µL |
| Composition | Synthetic linear double-stranded DNA in 10 mM Tris-HCl pH 7.5, 1 mM EDTA, 10 mM NaCl, 6% glycerol, 0.05% xylene cyanol FF, 0.05% bromophenol blue |
| Recommended loading | 5-6 µL (0.5-0.6 µg) per lane |
| Recommended gel | 1.0-1.5% agarose, optimal run distance 60-80 mm |
| Storage | -20 °C; aliquot and avoid repeated freeze-thaw cycles (more than 10 damage the marker) |
| Shipping | On wet ice |
| Stability | Very stable as supplied at -20 °C |
| Quality control | Exact size of every band, no ambiguous bands |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.