DNA ladders, loading buffers and gel stains
GenLadder 100 bp Plus DNA ladder with gel staining dye (ready-to-use)
DNA ladder from 100 bp to 3 kb with built-in gel stain, no pre- or post-staining
- Cat. No.
- M3237
- Formats
- 2 formats
- Manufacturer
A ready-to-use synthetic ladder that contains, besides the Orange G and xylene cyanol FF tracking dyes, a non-toxic fluorescent nucleic acid stain: load it on unstained agarose gels and the bands become visible without any extra staining. It covers 100 to 1,000 bp in 100 bp steps, with two extra bands at 1,500 and 3,000 bp, and the 500 and 1,500 bp bands are more intense. Fluorescence is much stronger under blue-light excitation, which suits high-throughput workflows and colony screening.
Key features
Built-in stain
No gel pre- or post-staining: the ladder carries its own fluorescent stain.
Safe to use
Non-toxic, non-mutagenic stain that can go into normal waste in line with local rules.
Optimised for blue light
Excitation at about 490 nm; a small UV excitation peak also allows reading on UV transilluminators, with a weaker signal.
Reference bands
500 and 1,500 bp more intense for quick orientation.
Applications
- Molecular weight standard for DNA electrophoresis in unstained gels
- Colony screening
- High-throughput analysis of PCR products
- Use alongside master mixes that contain a gel stain
Technical details
| Range | 100-3,000 bp |
|---|---|
| Bands | 12: 100 to 1,000 bp in 100 bp steps, plus 1,500 and 3,000 bp; 500 and 1,500 bp more intense |
| Concentration | 0.09 µg/µL (90 µg/mL) |
| Composition | Synthetic blunt-ended linear double-stranded DNA fragments, tracking dyes and a fluorescent nucleic acid stain |
| Tracking dyes | Orange G and xylene cyanol FF |
| Recommended loading | 5-6 µL (0.5-0.6 µg) per lane on an unstained gel |
| Detection | Blue light (about 490 nm), recommended; UV also possible with a lower signal |
| Storage | -20 °C for up to 1 year; +2/+8 °C for up to 6 months; avoid repeated freeze-thaw cycles |
| Shipping | On wet ice |
| Quality control | Exact size of every band, no ambiguous bands |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.