DNA ladders, loading buffers and gel stains
GenLadder XLarge up to 25 kbp (ready-to-use)
Ready-to-use DNA ladder from 250 bp to 25 kb, with reference bands at 1,000 and 3,000 bp
- Cat. No.
- GM3084
- Formats
- 2 formats
- Manufacturer
A synthetic molecular weight marker for sizing large DNA fragments in agarose gels, from 250 to 25,000 bp. It contains 14 bands made from restriction-digested plasmids and PCR products, and the bands at 1,000 and 3,000 bp are stronger to serve as reference points. It comes premixed with loading buffer and tracking dyes, so it goes straight into the well.
Key features
Ready to use
Premixed with loading buffer, bromophenol blue and xylene cyanol FF: load 5-6 µL per lane.
Wide range
14 fragments from 250 bp to 25 kb for sizing plasmid inserts and digested vectors.
Reference bands
The double bands at 1,000 and 3,000 bp are brighter than the others for quick orientation.
Exact sizes
Each band matches its stated size, with no ambiguous bands.
Applications
- Molecular weight standard for agarose gel electrophoresis of DNA
- Sizing of plasmid inserts
- Checking restriction-digested plasmid vectors
Technical details
| Range | 250-25,000 bp |
|---|---|
| Bands | 14: 250, 500, 750, 1,000 (double), 1,500, 2,000, 2,500, 3,000 (double), 4,000, 5,000, 6,000, 8,000, 10,000, 25,000 bp |
| Concentration | About 0.1 µg/µL |
| Composition | Linear double-stranded DNA in 10 mM Tris-HCl pH 8.0, 10 mM EDTA, with bromophenol blue and xylene cyanol FF |
| Recommended loading | 5-6 µL (0.5-0.6 µg) per lane |
| Recommended gel | 1.0-1.5% agarose, optimal run distance 60-80 mm |
| Storage | -20 °C; aliquot and avoid repeated freeze-thaw cycles (more than 10 damage the marker) |
| Shipping | On wet ice |
| Stability | Very stable as supplied at a constant -20 °C |
| Quality control | Exact size of every band, no ambiguous bands |
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.