Dehydrated culture media
Liver Agar
For the cultivation of Brucella and other pathogenic organisms.
- Cat. No.
- LC-1142
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Liver Agar is used to grow Brucella and other fastidious pathogens in foods. Whilst most strains of Brucella will grow on chocolate or blood agar, this medium is preferred due to the high nutrient base permitting an extensive growth of Brucella and other fastidious pathogens.
Key features
Applications
Enrichment of Brucella.
Industry
Food.
Preparation
41.4 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
The growth of most anaerobic bacteria is promoted by the growth nutrients and stimulants such as nitrogen, vitamins, minerals and amino acids contained in the Liver Agar. Dextrose is the source of fermentable carbohydrate for carbon and energy. In low concentrations, starch absorbs any toxic metabolites produced. Bacteriological agar is the solidifying agent.
Brucellosis is a zoonotic disease with a domestic animal reservoir. The usual route of transmission is by milk, milk products, meat and direct contact with infected animals.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.6 ± 0.2 |
| Amount per litre | 41.4 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 11 |
| Dextrose | 0.2 |
| Starch | 0.2 |
| Liver base | 30 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 41.4 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Inoculate and incubate the medium at 35 ± 2 °C under 5-10% CO₂ or anaerobic conditions for 18-48 hours and up to 72 hours if necessary.
- For isolating Brucella from contaminated milk, crystal violet can be added to suppress Gram-positive organisms. Addition of 5% heated horse or rabbit serum enhances the growth of Brucella.
Quality control
Microbiological test
Incubation conditions (Streptococcus pyogenes): 35 ± 2 °C / 5-10% CO₂ / 18-48 h.
Incubation conditions (Clostridium sporogenes): 35 ± 2 °C / anaerobic conditions / 18-48 h.
The microbiological test of the Brucella species should be carried out by the end-user laboratory.
| Microorganism | Specification |
|---|---|
| Clostridium sporogenesATCC 11437 | Good growth |
| Streptococcus pyogenesATCC 19615 | Good growth |
Bibliography
- Scarr M. Pamela (1958) DSIR Proc. Symp Found Microbiol 1957 HMSO London pp 29-33
What you receive
The product
Shipped under controlled temperature when the product requires it.
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