Dehydrated culture media
PPLO Agar Base w/o Crystal Violet
For the isolation and culture of PPLO microorganisms: Mycoplasma.
- Cat. No.
- LC-1140
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
PPLO Agar Base w/o Crystal Violet is used in the study of the growth requirements of Mycoplasma, along with the identification and cultivation of this organism. PPLO stands for Pleuro Pneumonia-Like Organisms and was described by Morton, Smith and Leberman.
Key features
Applications
Selective enrichment and isolation of Mycoplasma.
Preparation
35 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Peptone and beef heart infusion provide the nutrients for growth: nitrogen, vitamins, minerals and amino acids, whilst the sodium chloride provides the osmotic balance. Crystal violet is not included in this formula since it is inhibitory to some Mycoplasma. Bacteriological agar is the solidifying agent.
PPLO colonies have a round shape and a dense center with a less dense periphery, giving a "fried egg" appearance on agar.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.8 ± 0.2 |
| Amount per litre | 35 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 14 |
| Beef heart infusion | 6 |
| Peptone | 10 |
| Sodium chloride | 5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 35 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C and, if desired, aseptically add 1% serum fraction or 25% ascitic fluid. Homogenize gently and dispense into Petri dishes.
Instructions for use
Inoculation method:
- Grow the microorganism in PPLO Broth Base w/o Crystal Violet (LC-1262).
- Inoculate the test sample in PPLO Agar w/o Crystal Violet with 0.1 mL of serial dilutions. Use the pour plate method or the swab-inoculation technique.
- Incubate at 35 ± 2 °C with 5-10% CO₂, for a maximum of 7 days.
Quality control
The microbiological test should be carried out by the end-user laboratory.
Bibliography
- Adler, H.E. and AJ Da Massa. 1967 Use of formalinized Mycoplasma gallisepticum antigens and chicken erythrocytes in hemagglutination and hemagglutination-inhibition studies. Appl. Microbiol 15:245-248.
- Morton HE and JG Lecce. 1953. Selective action of thallium acetate and crystal violet for pleuropneumonia like organisms of human origin. J. Bacteriol 66:646-649.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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