Dehydrated culture media
PPLO Broth Base w/o Crystal Violet
For the enrichment of PPLO microorganisms. Mycoplasma in mixed cultures.
- Cat. No.
- LC-1262
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
PPLO Broth Base w/o Crystal Violet is used in the studies of the growth requirements of Mycoplasma, along with the identification and cultivation of this organism. PPLO Broth w/o Crystal Violet is prepared according to the formula described by Morton and Lecce.
Key features
Applications
Selective enrichment of Mycoplasma.
Preparation
21 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Peptone and beef heart infusion provide nitrogen, vitamins, minerals and amino acids essential for growth, whilst the sodium chloride provides the osmotic balance. Crystal violet is not included in this formula since it is inhibitory on some Mycoplasma.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber |
| Final pH (25 °C) | 7.8 ± 0.2 |
| Amount per litre | 21 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Beef heart infusion | 6 |
| Peptone | 10 |
| Sodium chloride | 5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 21 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C and, if desired, aseptically add supplements and additives. Homogenize gently.
Instructions for use
- Inoculate and incubate at 35 ± 2 °C under 5-10% CO₂ for 24-72 hours.
- Subculture to PPLO Agar (LC-1140) and incubate at 35 ± 2 °C under 5-10% CO₂ for up to 7 days.
- Examine daily for growth.
Quality control
The microbiological test should be carried out by the end-user laboratory.
Bibliography
- Leland DS, MA Lapworth, RB Jones and MLV French 1982. Comparative evaluation of media for isolation of Ureaplasma urealyticum and genital Mycoplasma species. J. Clin. Microbiol. 16:709-714.
- Kenny GE 1985 Mycoplasmas, p. 407-411 In EH Lennette, A Balows Manual of clinical microbiology, 4th ed. American Society for Microbiology, Washington DC.
- Morton and Lecce. 1953. J. Bacteriol. 66:646.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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