Dehydrated culture media
Yeast Extract Soy Broth (TSB no animal)
For general laboratory use and to cultivate fastidious microorganisms.
- Cat. No.
- LC-1268
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Yeast Extract Soy Broth (TSB no animal), a general purpose medium, is similar to Trypticasein Soy Broth but with all its components of vegetable origin. It is a medium rich in nutrients for general use in microbiological laboratories. It supports the abundant growth of fastidious microorganisms such as pneumococci, streptococci, Neisseriae etc.
Key features
Applications
Diluent and enrichment for fastidious microorganisms and for general use.
Industry
Cosmetics, food, pharmaceutical/veterinary.
Preparation
30 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Soy peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Dipotassium phosphate acts as a buffer system.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 30 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 2.5 |
| Dipotassium phosphate | 2.5 |
| Sodium chloride | 5 |
| Soy peptone | 11 |
| Yeast extract | 8.8 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 30 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
- Take the inoculum with a sterile loop.
- Submerge the loop into the medium and shake gently.
- Incubate at 35 ± 2 °C for 18-48 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-48 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Aspergillus brasiliensisATCC 16404 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
| Streptococcus pneumoniaeATCC 6305 | Good growth |
| Staphylococcus aureusATCC 6538 | Good growth |
| Bacillus subtilisATCC 6633 | Good growth |
Bibliography
- Gibbons and McDonald. J. Bacteriol., 80:164. 1960.
- Havens and Benham. A. Med. Tech., 23:305. 1957.
- Muey and Edward. Proc. Soc. Exper. Biol. and Med., 97:550. 1958.
- Steward and Kelly. J. Bacteriol., 77:101. 1959.
- MacFaddin, J.D. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria, p. 797. vol. 1. Williams & Wilkins, Baltimore, MD.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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