Dehydrated culture media
Potato Dextrose Broth
For the cultivation of yeasts and molds.
- Cat. No.
- LC-1261
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Potato Dextrose Broth is a liquid medium used for cultivating yeasts and molds. It can be used for the growth of clinically significant yeasts and molds from foods and dairy products.
Key features
Applications
Selective enrichment of yeasts and molds.
Preparation
26.5 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
This general purpose medium can be supplemented with acid or antibiotics to inhibit bacterial growth. The nutritionally rich base (potato infusion) encourages a very rich fungal and mold growth. Dextrose is the fermentable carbohydrate as carbon and energy source. The low pH of this medium inhibits bacterial growth.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 5.6 ± 0.2 |
| Amount per litre | 26.5 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 20 |
| Infusion from potatoes | 6.5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 26.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Inoculate the medium and incubate at 25-30 °C for 48-72 hours. Growth is indicated as turbidity.
Inoculation of Potato Dextrose Broth with pure cultures of yeasts can assist in their identification. Observe cultures for surface growth and pellicle formation. Perform microscopic examination and biochemical tests to identify isolates to genus and species if necessary.
Quality control
Microbiological test
Incubation conditions: 25-30 °C / 48-72 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Aspergillus brasiliensisATCC 16404 | Good growth |
| Saccharomyces cerevisiaeATCC 9763 | Good growth |
Bibliography
- Association of Official Analytical Chemists. 1995. Bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, MD.
- MacFaddin, J.F. 1985. Media for isolation-cultivation-identification-maintenance of medical bacteria, vol. 1. Williams & Wilkins, Baltimore, MD.
- Frank, J.F., G.L. Christen, and L.B. Bullerman (G.H. Richardson, Tech. Comm.) 1993. Tests for groups of microorganisms. P. 271-286. In Marshall, R.T. (ed.). Standard methods for the microbiological examination of dairy products, 16th ed. American Public Health Association, Washington, D.C.
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The product
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