Dehydrated culture media
FMM Broth
For the cultivation of Flexibacter maritimus.
- Cat. No.
- LC-1264
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
FMM Broth is a medium used for the cultivation of Tenacibaculum maritimum (formerly Flexibacter maritimus), which is responsible for Flexibacteriosis marina, one of the most important bacterial diseases affecting marine life around the world. This has an important economic impact on aquaculture producers. The infection has a variety of clinical manifestations depending on the species and age of fish, the most significant symptom being the presence of gross lesions on the body surface. It is currently the main pathological problem in turbot and sole cultivation, affecting all ages of fish.
Key features
Applications
Selective isolation of Flexibacter maritimus.
Industry
Pharmaceutical/veterinary.
Preparation
36.7 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly of the B-group. The rest of the components provide the salinity requirements that make the medium similar to sea water.
The isolated bacteria are biochemically characterized by conventional methods in plates and tubes. They are Gram-negative filamentous bacilli, oxidase and catalase positive, nitrate reducers and incapable of producing sulphydric acid. They are negative for the test of indole and methyl red. To confirm the taxonomic position, perform molecular and serological tests (PCR).
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Whitish |
| Color of the prepared medium | Light amber |
| Final pH (25 °C) | 7.4 ± 0.2 |
| Amount per litre | 36.7 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Ammonium nitrate | 0.0016 |
| Boric acid | 0.022 |
| Calcium chloride | 1.8 |
| Casein peptone | 5 |
| Disodium phosphate | 0.008 |
| Potassium bromide | 0.08 |
| Potassium chloride | 0.55 |
| Sodium acetate | 0.01 |
| Sodium bicarbonate | 0.16 |
| Sodium chloride | 19.4 |
| Sodium fluoride | 0.0024 |
| Sodium silicate | 0.004 |
| Sodium sulfate | 3.24 |
| Strontium chloride | 0.034 |
| Yeast extract | 0.5 |
| Magnesium chloride | 5.9 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 36.7 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Incubate at 25 °C for 72 hours.
Quality control
Microbiological test
Incubation conditions: 25 °C / 72 h.
| Microorganism | Specification |
|---|---|
| Tenacibaculum maritimumATCC 43397 | Good growth |
Bibliography
- Dinis, M.T., Ribeiro, L., Soares, F. & Sarasquete, C. (1999) A review on the cultivation potential of Solea senegalensis in Spain and in Portugal. Aquaculture 176, 27-38.
- Dopazo, C.P. & Barja, J.L. (2002) Diagnosis and identification of IPNV in salmonids by molecular methods. In: Molecular Diagnosis of Salmonid Diseases. pp. 23-48. C. Cunningham (ed). Kluwer Academic Publishers. Dordrecht, Holland.
- Osorio, C. and Toranzo, A.E. 2002. DNA-based diagnostics in Sea Farming. In: R. Nagabhushanam and M. Fingerman (Editors), Recent Advances in Marine Biotechnology Series Vol. 7: Seafood safety and Human Health. Science Publishers, Inc. Plymouth, UK. pp. 253-310.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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