Dehydrated culture media
Glucose Chloramphenicol Broth
Selective medium for the isolation and enumeration of yeasts and molds in milk and dairy products using the MPN technique.
- Cat. No.
- LC-1258
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Glucose Chloramphenicol Broth is recommended by the International Dairy Federation (FIL-IDF) for the isolation and enumeration of yeasts and molds in milk and dairy products, using the most probable number (MPN) method.
Key features
Applications
Selective enumeration and selective isolation of yeasts and molds.
Industry
Dairy products.
Preparation
25.2 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Yeast extract is the water-soluble portion of hydrolyzed yeast and is a source of vitamins, particularly of the B-group, and other growth nutrients that stimulate yeast and mold development. Glucose is the fermentable carbohydrate as a carbon and energy source. Chloramphenicol is an antibiotic which aids in isolating pathogenic fungi from heavily contaminated material, as it inhibits most contaminating bacteria. It is a recommended antibiotic for use with media due to its heat stability and wide bacterial spectrum.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Light amber |
| Final pH (25 °C) | 6.6 ± 0.2 |
| Amount per litre | 25.2 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Glucose | 20 |
| Chloramphenicol | 0.2 |
| Yeast extract | 5 |
Preparation and use
Preparation
Suspend 25.2 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Inoculate with a series of dilutions as indicated in the MPN technique. Incubate at 25-30 °C and examine after 3-7 days. Report as number of colonies per gram of food.
Quality control
Microbiological test
Incubation conditions: 25-30 °C / 3-7 days.
| Microorganism | Specification |
|---|---|
| Aspergillus spp. | Good growth |
| Candida albicansATCC 2091 | Good growth |
| Escherichia coliATCC 25922 | Inhibited growth |
| Staphylococcus aureusATCC 25923 | Inhibited growth |
| Lactobacillus rhamnosusATCC 9595 | Inhibited growth |
Bibliography
- FIL-IDF (1991) Standard 94B. Enumeration of yeast and moulds. Colony Count Technique at 25 °C.
- ISO (1981) ISO/DIS 6611: Milk and Milk products: Enumeration of yeast and moulds colony count technique at 25 °C.
- DIN Standard 10186. Mikrobiologische Milchuntersuchung. Bestimmung der Anzahl von Hefen und Schimmelpilzen.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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Frequently asked questions
Can I ask for formats other than those listed?
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