Dehydrated culture media
Nutrient Broth No.2
For the cultivation of fastidious pathogens and other microorganisms.
- Cat. No.
- LC-1251
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Nutrient Broth No. 2 is used for the general cultivation of a wide variety of microorganisms. It is also used as a general use medium, rich in nutrients, that allows the growth of bacteria when there is a low quantity of inocula and of fastidious pathogenic microorganisms. It can also be used for sterility testing of aerobic organisms.
Key features
Applications
Enrichment of fastidious microorganisms.
Industry
General cultivation.
Preparation
25 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
The medium is particularly suitable for subculture, especially as a secondary growth medium for staphylococci to be tested for coagulase production.
Gelatin peptone and beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.5 ± 0.2 |
| Amount per litre | 25 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Casein peptone | 10 |
| Beef extract | 10 |
| Sodium chloride | 5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 25 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Inoculate the medium with the test sample and incubate at 35 ± 2 °C for 18-24 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-48 h.
| Microorganism | Specification |
|---|---|
| Staphylococcus epidermidisATCC 12228 | Good growth |
| Klebsiella aerogenesATCC 13048 | Good growth |
| Salmonella typhimuriumATCC 14028 | Good growth |
| Streptococcus pyogenesATCC 19615 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
Bibliography
- Walsbren, Carr, and Dunnette A. J. Clin. Path. 21:884. 1951.
- American Public Health Association. 1923. Standard methods of water analysis, 5th ed. American Public Health Association, Washington, D.C.
- Marshall, R.T. (ed) 1993. Standard methods for the microbiological examination of dairy products, 16th ed. American Public Health Association, Washington, D.C.
What you receive
The product
Shipped under controlled temperature when the product requires it.
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