Dehydrated culture media

Malt Extract Broth

For the isolation and enumeration of molds and yeast.

Cat. No.: LC-1245
Category: Dehydrated culture media
Format: 500 g
Storage: 2 to 25 °C
Minimum guaranteed shelf life: 15 months

Malt Extract Broth is used for the isolation, cultivation and enumeration of yeasts and molds from foods.

Key features

Applications

Selective enumeration of yeasts and molds.

Industry

Food.

Preparation

19 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 118 °C for 15 minutes.

Principles and uses

Malt extract provides the carbon, protein and nutrient sources required for the growth of microorganisms. It is particularly suitable for yeasts and molds as it contains a high concentration of maltose and other saccharides as energy sources. Yeast extract is the source of vitamins, particularly of the B-group. The acidic pH of the Malt Extract Broth is optimum for the growth of yeasts and molds whilst restricting other bacterial growth.

Technical specifications

Solubility	Without residues
Appearance	Fine powder
Color of the dehydrated medium	Beige
Color of the prepared medium	Amber, slightly opalescent
Final pH (25 °C)	4.7 ± 0.2
Amount per litre	19 g
Storage	2 to 25 °C

Formula

Ingredient	g/L
Gelatin peptone	5.5
Malt extract	13
Yeast extract	0.5

Preparation and use

Preparation

Suspend 19 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 118 °C for 15 minutes. DO NOT OVERHEAT.

Instructions for use

Use standard procedures to inoculate the sample material into the Malt Extract Agar tubes.
Incubate the tubes at 30 ± 2 °C for 18-72 hours.

Quality control

Microbiological test

Incubation conditions: 30 ± 2 °C / 18-72 h.

Microorganism	Specification
Candida albicansATCC 10231	Good growth
Aspergillus brasiliensisATCC 16404	Good growth
Saccharomyces cerevisiaeATCC 9080	Good growth
Saccharomyces cerevisiaeATCC 9763	Good growth

Bibliography

Thom and Raper, Manual of the Aspergilli 39:1945

What you receive

The product

Shipped under controlled temperature when the product requires it.

Lot certificate

Every lot comes with its certificate.

Tracked shipment

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Frequently asked questions

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