Dehydrated culture media
Tryptose Phosphate Broth
For the cultivation of fastidious microorganisms.
- Cat. No.
- LC-1243
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Tryptose Phosphate Broth is recommended for the cultivation of fastidious and pathogenic microorganisms.
Key features
Applications
Enrichment of fastidious microorganisms.
Preparation
29.5 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Peptones provide nitrogen, vitamins, minerals and amino acids essential for growth. Dextrose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. The buffering capacity is provided by the disodium phosphate.
The addition of 0.1-0.2% agar to Tryptose Phosphate Broth delays the dispersion of CO₂ and diffusion of O₂ and facilitates the anaerobic growth. The low concentration of agar provides conditions for both aerobic growth in the upper zone and microaerophilic and anaerobic growth in the lower zone.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 29.5 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Dextrose | 2 |
| Casein peptone | 13 |
| Disodium phosphate | 2.5 |
| Sodium chloride | 5 |
| Proteose peptone No. 3 | 7 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 29.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
Inoculate and incubate at 35 ± 2 °C for 18-48 hours.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-48 h.
| Microorganism | Specification |
|---|---|
| Staphylococcus epidermidisATCC 12228 | Good growth |
| Neisseria meningitidisATCC 13090 | Good growth |
| Streptococcus pyogenesATCC 19615 | Good growth |
| Streptococcus pneumoniaeATCC 6305 | Good growth |
Bibliography
- Gray, M.L., Stafseth, H.J., a. Thorp, F. Jr.: The use of potassium tellurite, sodium azide and acetic acid in a selective medium for the isolation of Listeria monocytogenes. - J. Bact., 59: 443-444 (1950).
- Hausler, W.J., a. Koontz, F.P.: Brucellosis in Diagnostic procedures for Bacterial, Mycotic and Parasitic Infections: ed., APHA, New York (1970).
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
Other products in the same category
Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
How do I see the prices?
Prices are shown after you sign in to the customer area, with your account discount already applied.
How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.