Dehydrated culture media
Thioglycollate Broth NIH
For sterility assays of biological and pharmaceutical products.
- Cat. No.
- LC-1241
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Standards
- NIH
Thioglycollate Broth is prepared according to the formula of the National Institute of Health (NIH) and the United States Pharmacopoeia (USP). It is used in detecting microorganisms in normally sterile materials, and is an alternative to certain products that are turbid or cannot readily culture because of the viscosity.
Key features
Applications
Diluent (general use).
Industry
Pharmaceutical/veterinary.
Standards
NIH.
Preparation
28.5 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Casein peptone provides nitrogen, vitamins, minerals and amino acids essential for growth. Yeast extract is a source of vitamins, particularly the B-group. Sodium thioglycollate and L-cystine lower the oxidation-reduction potential by removing oxygen to maintain a low Eh. Dextrose is the carbohydrate energy source and allows for a rapid and vigorous growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance.
Technical specifications
| Color of the dehydrated medium | Beige |
|---|---|
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.1 ± 0.2 |
| Amount per litre | 28.5 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Casein peptone | 15 |
| L-Cystine | 0.5 |
| Sodium chloride | 2.5 |
| Sodium thioglycollate | 0.5 |
| Yeast extract | 5 |
| Dextrose anhydrous | 5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 28.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes. The medium does not contain agar or resazurin and it is preferable that it is freshly prepared, eliminating any dissolved oxygen before use by heating in boiling water or in a water bath.
Instructions for use
- Inoculate and incubate at 35 ± 2 °C for 18-48 hours.
- Anaerobic conditions can also be used with this medium.
Quality control
Microbiological test
Incubation conditions: 35 ± 2 °C / 18-48 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth, turbidity |
| Clostridium sporogenesATCC 19404 | Good growth, turbidity |
| Streptococcus pyogenesATCC 19615 | Good growth, turbidity |
| Bacteroides fragilisATCC 25285 | Good growth, turbidity |
| Escherichia coliATCC 25922 | Good growth, turbidity |
| Bacillus subtilisATCC 6633 | Good growth, turbidity |
Bibliography
- U.S. Pharmacopoeia XVI, 1960
What you receive
The product
Shipped under controlled temperature when the product requires it.
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