Dehydrated culture media
Malt Extract Agar
For the cultivation, isolation, and enumeration of fungi and yeast.
- Cat. No.
- LC-1038
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Also available
- Ready-to-use medium, 1 format (LC-827)
Malt Extract Agar is used for the isolation, cultivation and enumeration of yeasts and molds from foods.
Key features
Applications
Selective enumeration and selective isolation of yeasts and molds.
Preparation
33.6 g in one liter of distilled water; boil for one minute, do not overheat, sterilize in autoclave at 118 °C for 10 minutes.
Principles and uses
It is particularly suitable for yeasts and molds as it contains a high concentration of maltose and other saccharides as energy sources. Dextrin and glycerin are the carbon sources, and peptone is a nitrogen source. Bacteriological agar is the solidifying agent. The acidic pH of the Malt Extract Agar is optimum for the growth of yeasts and molds whilst restricting other bacterial growth.
Malt Extract Agar has been used for years to cultivate fungi and yeast cultures in the sugar industry, in the manufacturing of syrups, soft drinks, and other drinks.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 4.7 ± 0.2 |
| Amount per litre | 33.6 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 15 |
| Glycerol | 2.35 |
| Maltose | 12.75 |
| Peptone | 0.78 |
| Dextrin | 2.75 |
Preparation and use
Preparation
Suspend 33.6 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. DO NOT OVERHEAT. Sterilize in autoclave at 118 °C for 10 minutes. Cool to 45-50 °C, mix well and dispense into plates.
Instructions for use
Quality control
Microbiological test
Incubation conditions: 30 ± 2 °C / 18-72 h.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Aspergillus brasiliensisATCC 16404 | Good growth |
| Saccharomyces cerevisiaeATCC 9080 | Good growth |
| Saccharomyces cerevisiaeATCC 9763 | Good growth |
Bibliography
- Thom and Raper, Manual of the Aspergilli 39:1945
What you receive
The product
Shipped under controlled temperature when the product requires it.
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Frequently asked questions
Can I ask for formats other than those listed?
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