Dehydrated culture media
Yeast Extract Agar for Molds
For the cultivation of yeast and molds from diverse materials, especially milk and dairy products.
- Cat. No.
- LC-1312
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Yeast Extract Agar for Molds is suitable to cultivate molds and yeasts from milk and dairy products.
Key features
Applications
Growth of yeasts and molds.
Industry
Dairy products.
Preparation
35 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
Yeast extract is a source of vitamins, particularly of the B-group, essential for bacterial growth. Dextrose is the carbohydrate energy source. Bacteriological agar is the solidifying agent. The high dextrose concentration makes this medium selective for yeasts and molds.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 6.5 ± 0.2 |
| Amount per litre | 35 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 20 |
| Dextrose | 10 |
| Yeast extract | 5 |
Preparation and use
Preparation
Suspend 35 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50 °C, mix well and dispense into plates.
Instructions for use
The inoculation method can be carried out either by flooding or on the surface, depending on the purpose for which the medium is intended. Incubation time is 7 days at a temperature of 28 °C and in an aerobic environment.
For increased selectivity, acidify the medium or add antibiotics with the aseptic technique. It is not recommended to heat the acidified agar medium.
Quality control
Microbiological test
Incubation conditions: 28 °C / 7 days.
| Microorganism | Specification |
|---|---|
| Candida albicansATCC 10231 | Good growth |
| Aspergillus brasiliensisATCC 16404 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
| Staphylococcus aureusATCC 25923 | Good growth |
Bibliography
- Cooke, W.B. and A.R. Brazis. 1968. Occurrence of molds and yeasts in dairy products. Mycopathol. Mycol. Appl. 35:281-289.
- Overcast, W.W. and D.J. Weakley. 1969. An aureomycin-rose Bengal agar for enumeration of yeast and mold in cottage cheese.
- International Dairy Federation. Standard Method ISO/DIS 6611.
- Koburger, J.A. 1970. Fungi in foods: 1. Effect of inhibitor and incubation temperature on enumeration. J. Milk Food Technol. 33:433-434.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
Every lot comes with its certificate.
Tracked shipment
Follow your shipment live, from our warehouse to your lab.
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Frequently asked questions
Can I ask for formats other than those listed?
Yes: custom formats and OEM packs are available on request. Tell us the quantities you need.
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How long does delivery take?
We give you the availability with the quote, within 24 hours of your request.