Dehydrated culture media
Nutrient Agar Dev Regulations
For the enumeration of microorganisms in water and other materials.
- Cat. No.
- LC-1314
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
Nutrient Agar Dev Regulations is a general purpose medium, not selective but suitable for the cultivation of a wide variety of microorganisms.
Key features
Applications
Non-selective enumeration (general use).
Industry
Dairy products, water.
Preparation
43 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
It is recommended by the German Standard Methods (Deutsche Einheitsverfahren), the German Drinking Water Regulations (Trinkwasser-Verordnung) (1990) and the German Regulation for Food Examination (LMBG).
The American Public Health Association (APHA) suggested this standard culture medium for use in bacterial processing for water analysis. In Standard Methods of Water Analysis and Standard Methods of Milk Analysis, the APHA advocated the use of dehydrated media for the bacterial examination of water and milk.
Meat peptone and beef extract provide nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bacteriological agar is the solidifying agent.
Since this medium contains sodium chloride, it can be used as a base for enrichment with blood or other supplements for cultivating fastidious microorganisms.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Amber, slightly opalescent |
| Final pH (25 °C) | 7.3 ± 0.2 |
| Amount per litre | 43 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 18 |
| Beef extract | 10 |
| Meat peptone | 10 |
| Sodium chloride | 5 |
Preparation and use
Preparation
Suspend 43 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45 °C, mix well and dispense into plates.
Instructions for use
Inoculate the medium with the test sample and incubate at 20 ± 2 °C or 35 ± 1 °C for 44 ± 4 hours.
Quality control
Microbiological test
Incubation conditions: 20 ± 2 °C or 35 ± 1 °C / 44 ± 4 h.
| Microorganism | Specification |
|---|---|
| Enterococcus faecalisATCC 11700 | Good growth |
| Proteus vulgarisATCC 13315 | Good growth |
| Klebsiella pneumoniaeATCC 13883 | Good growth |
| Salmonella typhimuriumATCC 14028 | Good growth |
| Escherichia coliATCC 25922 | Good growth |
Bibliography
- German Standard Methods (Deutsche Einheitsverfahren), the German Drinking Water Regulations (Trinkwasser-Verordnung) (1990) and the German regulation for food examination (LMBG).
- American Public Health Association. 1923. Standard methods of milk analysis, 4th ed. American Public Health Association, Washington, D.C.
- Association of Official Analytical Chemists. 1995. Official methods of analysis of AOAC International, 16th ed. AOAC International, Arlington, VA.
What you receive
The product
Shipped under controlled temperature when the product requires it.
Lot certificate
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Tracked shipment
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