Dehydrated culture media
BCP Glucose Agar
For the confirmation of Enterobacteriaceae and Pseudomonas.
- Cat. No.
- LC-1320
- Category
- Dehydrated culture media
- Format
- 500 g
- Storage
- 2 to 25 °C
- Minimum guaranteed shelf life
- 15 months
- Standards
- ISO 11059ISO 11133ISO 21528
BCP Glucose Agar is used for the differentiation of Enterobacteriaceae in urine, water and food. It differentiates species on the basis of dextrose fermentation.
Key features
Applications
Confirmation of enterobacteria and Pseudomonas.
Industry
Dairy products, food, water.
Standards
ISO 11059, ISO 11133, ISO 21528.
Preparation
41.5 g in one liter of distilled water; boil for one minute, sterilize in autoclave at 121 °C for 15 minutes.
Principles and uses
This medium is recommended by ISO 11059 for the confirmation of Pseudomonas spp. in milk and milk products. BCP Glucose Agar was also recommended by ISO 21528:2014 for the confirmation of Enterobacteriaceae.
Tryptone and yeast extract provide nitrogen, vitamins, minerals and amino acids essential for growth. D-glucose is the fermentable carbohydrate providing carbon and energy. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Bromocresol purple is a pH indicator. Bacteriological agar is the solidifying agent.
The glucose-fermenting microorganisms produce yellow colonies (acid) and the non-fermenting ones, purple colonies. Colonies that are oxidase-negative and glucose-positive are confirmed as Enterobacteriaceae.
Technical specifications
| Solubility | Without residues |
|---|---|
| Appearance | Fine powder |
| Color of the dehydrated medium | Beige |
| Color of the prepared medium | Purple |
| Final pH (25 °C) | 7.0 ± 0.2 |
| Amount per litre | 41.5 g |
| Storage | 2 to 25 °C |
Formula
| Ingredient | g/L |
|---|---|
| Bacteriological agar | 15 |
| Bromocresol purple | 0.015 |
| D-Glucose | 10 |
| Sodium chloride | 5 |
| Tryptone | 10 |
| Yeast extract | 1.5 |
Typical formula in g/L, adjusted and/or supplemented as required to meet performance criteria.
Preparation and use
Preparation
Suspend 41.5 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Dispense into appropriate containers and sterilize in autoclave at 121 °C for 15 minutes.
Instructions for use
For the enumeration of Pseudomonas spp. in milk and milk products:
- Prepare the initial suspension and dilutions.
- Transfer 0.1 mL of the test sample or 0.1 mL of the initial suspension onto a PPA plate. Repeat with subsequent dilutions.
- Incubate the plates at 25 ± 1 °C for 48 ± 2 h.
- Streak each selected colony on Nutrient Agar plates for confirmation and incubate at 25 ± 1 °C for 24-48 h.
- For the confirmation, carry out the oxidase reaction and glucose fermentation tests.
- For the glucose fermentation assay, stab a colony from Nutrient Agar into tubes containing BCP Glucose Agar and incubate at 25 ± 1 °C for 24 ± 3 h without hermetically closing the tubes.
- Consider colonies with oxidase reaction (+) and glucose fermentation (-) as Pseudomonas colonies.
Quality control
According to ISO 11133
Incubation conditions: 25 ± 1 °C / 24 ± 3 h.
| Microorganism | Specification | Characteristic reaction |
|---|---|---|
| Pseudomonas fluorescensATCC 13525 | Good growth | No acid production (no color change to yellow) |
| Escherichia coliATCC 25922 | Good growth | Acid production (color change to yellow) |
| Pseudomonas aeruginosaATCC 27853 | Good growth | No acid production (no color change to yellow) |
Bibliography
- ISO/TS 11059 Milk and milk products - Method for the enumeration of Pseudomonas spp.
- ISO 21528-2:2004 Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 2: Colony-count method.
- Drigalsky, C. (1902) Über ein Verfahren zum Nachweis der typhusbacillen.
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